The impact of matrix age on intervertebral disc regeneration.
3D models
Ageing
Decellularization
IVD degeneration/regeneration
Tissue engineering
Journal
Biomaterials advances
ISSN: 2772-9508
Titre abrégé: Biomater Adv
Pays: Netherlands
ID NLM: 9918383886206676
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
received:
09
05
2022
revised:
26
09
2022
accepted:
03
11
2022
pubmed:
21
11
2022
medline:
30
11
2022
entrez:
20
11
2022
Statut:
ppublish
Résumé
With the lack of effective treatments for low back pain, the use of extracellular matrix (ECM)-based biomaterials have emerged with undeniable promise for IVD regeneration. Decellularized scaffolds can recreate an ideal microenvironment inducing tissue remodeling and repair. In particular, fetal tissues have a superior regenerative capacity given their ECM composition. In line with this, we unraveled age-associated alterations of the nucleus pulposus (NP) matrisome. Thus, the aim of the present work was to evaluate the impact of ECM donor age on IVD de/regeneration. Accordingly, we optimized an SDS (0.1 %, 1 h)-based decellularization protocol that preserves ECM cues in bovine NPs from different ages. After repopulation with adult NP cells, younger matrices showed the highest repopulation efficiency. Most importantly, cells seeded on younger scaffolds produced healthy ECM proteins suggesting an increased capacity to restore a functional IVD microenvironment. In vivo, only fetal matrices decreased neovessel formation, showing an anti-angiogenic potential. Our findings demonstrate that ECM donor age has a strong influence on angiogenesis and ECM de novo synthesis, opening new avenues for novel therapeutic strategies for the IVD. Additionally, more appropriate 3D models to study age-associated IVD pathology were unveiled.
Identifiants
pubmed: 36403438
pii: S2772-9508(22)00469-1
doi: 10.1016/j.bioadv.2022.213192
pii:
doi:
Substances chimiques
Extracellular Matrix Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
213192Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The co-authors Mário Adolfo Barbosa, Joana Caldeira, Raquel Madeira Gonçalves and Morena Francesca Fiordalisi, has a patent application for fetal decellularization procedure (WO2021118379 – 17.06.2021): “Fetal decellularized nucleus pulposus material and methods for obtaining pharmaceutic compositions to be used in the treatment of intervertebral disc degeneration and low back pain). Joana Rita Ferreira is currently an employee of AstraZeneca R&D, Gothenburg, Sweden. The remaining co-authors have no conflict of interest to declare.