Culture and characterization of canine and feline corneal epithelial organoids: A new tool for the study and treatment of corneal diseases.
canine
comparative ophthalmology
cornea
feline
organoid
stem cell
Journal
Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658
Informations de publication
Date de publication:
2022
2022
Historique:
received:
21
09
2022
accepted:
19
10
2022
entrez:
21
11
2022
pubmed:
22
11
2022
medline:
22
11
2022
Statut:
epublish
Résumé
In this study, we isolated and cultured canine and feline 3D corneal organoids. Samples derived from corneal limbal epithelium from one canine and one feline patient were obtained by enucleation after euthanasia. Stem cell isolation and organoid culture were performed by culturing organoids in Matrigel. Organoids were subsequently embedded in paraffin for further characterization. The expression of key corneal epithelial and stromal cell markers in canine and feline organoids was evaluated at the mRNA level by RNA-ISH and at the protein level by immunofluorescence (IF) and immunohistochemistry (IHC), while histochemical analysis was performed on both tissues and organoids using periodic-acid Schiff (PAS), Sirius Red, Gomori's Trichrome, and Colloidal Iron stains. IF showed consistent expression of AQP1 within canine and feline organoids and tissues. P63 was present in canine tissues, canine organoids, and feline tissues, but not in feline organoids. Results from IHC staining further confirmed the primarily epithelial origin of the organoids. Canine and feline 3D corneal organoids can successfully be cultured and maintained and express epithelial and stem cell progenitor markers typical of the cornea. This novel
Identifiants
pubmed: 36406087
doi: 10.3389/fvets.2022.1050467
pmc: PMC9672346
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1050467Informations de copyright
Copyright © 2022 Bedos, Wickham, Gabriel, Zdyrski, Allbaugh, Sahoo, Sebbag, Mochel and Allenspach.
Déclaration de conflit d'intérêts
Author KA is a co-founder of 3D Health Solutions. She serves as a consultant for Ceva Animal Health, Bioiberica, LifeDiagnostics, Antech Diagnostics, Deerland Probiotics, and Mars. Author JM is a co-founder of LifEngine Animal Health and 3D Health Solutions. He serves as a consultant for Ceva Animal Health and Ethos Animal Health. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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