Assessment of dd-cfDNA Levels in Clinically Stable Lung Allograft Recipients Beyond the Initial 2 y Posttransplant.


Journal

Transplantation direct
ISSN: 2373-8731
Titre abrégé: Transplant Direct
Pays: United States
ID NLM: 101651609

Informations de publication

Date de publication:
Dec 2022
Historique:
received: 25 08 2022
revised: 26 09 2022
accepted: 27 09 2022
entrez: 21 11 2022
pubmed: 22 11 2022
medline: 22 11 2022
Statut: epublish

Résumé

Donor-derived cell-free DNA (dd-cfDNA) is a useful biomarker for the diagnosis of acute allograft injury within the first 1 to 2 y after lung transplant, but its utility for diagnosing chronic lung allograft dysfunction (CLAD) has not yet been studied. Understanding baseline dd-cfDNA kinetics beyond the initial 2 y posttransplant is a necessary first step in determining the utility of dd-cfDNA as a CLAD biomarker. We seek to establish baseline dd-cfDNA% levels in clinically stable lung allograft recipients who are >2 y posttransplant. We performed a prospective, single-center, observational study to identify plasma dd-cfDNA levels in clinically stable lung allograft recipients >2 y posttransplant. Fifty-one subjects were enrolled and ≥3 baseline dd-cfDNA measurements were acquired during a median of 252 d. The median baseline percent dd-cfDNA level in our cohort was 0.45% (interquartile range [IQR], 0.26-0.69). There were statistically significant differences in dd-cfDNA based on posttransplant duration (≤5 y posttransplant median 0.41% [IQR, 0.21-0.64] versus >5 y posttransplant median 0.50% [IQR, 0.33-0.76]; This study is the first to define levels of dd-cfDNA in clinically stable patients who are >2 y post-lung transplant. These findings lay the groundwork for the study of dd-cfDNA as a possible biomarker for CLAD.

Identifiants

pubmed: 36406896
doi: 10.1097/TXD.0000000000001411
pmc: PMC9671749
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e1411

Subventions

Organisme : NHLBI NIH HHS
ID : R01 HL160551
Pays : United States

Informations de copyright

Copyright © 2022 The Author(s). Transplantation Direct. Published by Wolters Kluwer Health, Inc.

Références

J Appl Lab Med. 2017 Nov 1;2(3):309-321
pubmed: 33636851
J Appl Lab Med. 2022 Jun 30;7(4):901-909
pubmed: 35024828
Curr Opin Organ Transplant. 2020 Jun;25(3):268-273
pubmed: 32332197
J Thorac Dis. 2014 Aug;6(8):1039-53
pubmed: 25132971
J Heart Lung Transplant. 2021 Aug;40(8):822-830
pubmed: 34130911
J Thorac Dis. 2017 Aug;9(8):2650-2659
pubmed: 28932572
ERJ Open Res. 2021 Jan 25;7(1):
pubmed: 33532456
ERJ Open Res. 2020 Jun 15;6(2):
pubmed: 32577419
J Heart Lung Transplant. 2017 Sep;36(9):921-933
pubmed: 28662986
Am J Respir Crit Care Med. 2018 Dec 15;198(12):e116-e136
pubmed: 30640517
Thorax. 2017 Nov;72(11):1052-1054
pubmed: 28446663
J Biomed Inform. 2009 Apr;42(2):377-81
pubmed: 18929686
J Thorac Dis. 2017 Aug;9(8):2684-2691
pubmed: 28932576
Am J Transplant. 2022 Oct;22(10):2451-2457
pubmed: 35322546
Am J Transplant. 2008 Jul;8(7):1544-9
pubmed: 18557742
EBioMedicine. 2019 Feb;40:541-553
pubmed: 30692045
JAMA Surg. 2015 Mar 1;150(3):252-9
pubmed: 25629390
J Heart Lung Transplant. 2018 Jul;37(7):925-932
pubmed: 29500138
Clin Chem. 2020 Oct 1;66(10):1290-1299
pubmed: 33001185
J Heart Lung Transplant. 2020 Aug;39(8):761-770
pubmed: 32418864
Front Immunol. 2019 Jul 17;10:1681
pubmed: 31379869
J Heart Lung Transplant. 2014 Feb;33(2):127-33
pubmed: 24374027
J Biomed Inform. 2019 Jul;95:103208
pubmed: 31078660
Transplant Direct. 2022 Mar 25;8(4):e1317
pubmed: 35372675
Biophys Rev. 2020 Aug;12(4):917-924
pubmed: 32683591

Auteurs

Anil J Trindade (AJ)

Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN.
Vanderbilt Transplant Center, Vanderbilt University Medical Center, Nashville, TN.

Kaitlyn C Chapin (KC)

Vanderbilt Transplant Center, Vanderbilt University Medical Center, Nashville, TN.

Amy Mullican (A)

Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN.

Jennifer N Gray (JN)

CareDx, Inc, Brisbane, CA.

Haley Hoy (H)

Vanderbilt Transplant Center, Vanderbilt University Medical Center, Nashville, TN.

Caitlin T Demarest (CT)

Vanderbilt Transplant Center, Vanderbilt University Medical Center, Nashville, TN.
Department of Thoracic Surgery, Vanderbilt University Medical Center, Nashville, TN.

Eric S Lambright (ES)

Vanderbilt Transplant Center, Vanderbilt University Medical Center, Nashville, TN.
Department of Thoracic Surgery, Vanderbilt University Medical Center, Nashville, TN.

Katie A McPherson (KA)

Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN.
Vanderbilt Transplant Center, Vanderbilt University Medical Center, Nashville, TN.

Stephanie G Norfolk (SG)

Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN.
Vanderbilt Transplant Center, Vanderbilt University Medical Center, Nashville, TN.

Ivan M Robbins (IM)

Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN.
Vanderbilt Transplant Center, Vanderbilt University Medical Center, Nashville, TN.

Matthew Bacchetta (M)

Department of Thoracic Surgery, Vanderbilt University Medical Center, Nashville, TN.
Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, TN.
Department of Biomedical Engineering, School of Engineering, Vanderbilt University, Nashville, TN.

David B Erasmus (DB)

Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN.
Vanderbilt Transplant Center, Vanderbilt University Medical Center, Nashville, TN.

Ciara M Shaver (CM)

Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN.
Vanderbilt Transplant Center, Vanderbilt University Medical Center, Nashville, TN.

Classifications MeSH