A sample-to-answer COVID-19 diagnostic device based on immiscible filtration and CRISPR-Cas12a-assisted detection.
COVID-19
CRISPR-Cas
Diagnostics
Immiscible filtration
Point-of-care
SARS-CoV-2
Journal
Talanta open
ISSN: 2666-8319
Titre abrégé: Talanta Open
Pays: Netherlands
ID NLM: 9918315985806676
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
received:
22
09
2022
revised:
02
11
2022
accepted:
02
11
2022
entrez:
21
11
2022
pubmed:
22
11
2022
medline:
22
11
2022
Statut:
ppublish
Résumé
In response to the ongoing coronavirus disease 2019 (COVID-19) pandemic and disparities of vaccination coverage in low-and middle-income countries, it is vital to adopt a widespread testing and screening programme, combined with contact tracing, to monitor and effectively control the infection dispersion in areas where medical resources are limited. This work presents a lab-on-a-chip device, namely 'IFAST-LAMP-CRISPR', as an affordable, rapid and high-precision molecular diagnostic means for detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The herein proposed 'sample-to-answer' platform integrates RNA extraction, amplification and molecular detection with lateral flow readout in one device. The microscale dimensions of the device containing immiscible liquids, coupled with the use of silica paramagnetic beads and guanidine hydrochloride, streamline sample preparation (including RNA extraction, concentration and purification) in 15 min with minimal hands-on steps. The pre-amplification in combination with CRISPR-Cas12a detection assays targeting the nucleoprotein (N) gene achieved visual identification of ≥ 470 copies mL
Identifiants
pubmed: 36406953
doi: 10.1016/j.talo.2022.100166
pii: S2666-8319(22)00083-2
pmc: PMC9640297
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100166Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article
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