Micro-Topographies Induce Epigenetic Reprogramming and Quiescence in Human Mesenchymal Stem Cells.
biomaterials
epigenetics
mechanobiology
mesenchymal stem cells
nucleus
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
22 Nov 2022
22 Nov 2022
Historique:
revised:
19
09
2022
received:
08
07
2022
entrez:
22
11
2022
pubmed:
23
11
2022
medline:
23
11
2022
Statut:
aheadofprint
Résumé
Biomaterials can control cell and nuclear morphology. Since the shape of the nucleus influences chromatin architecture, gene expression and cell identity, surface topography can control cell phenotype. This study provides fundamental insights into how surface topography influences nuclear morphology, histone modifications, and expression of histone-associated proteins through advanced histone mass spectrometry and microarray analysis. The authors find that nuclear confinement is associated with a loss of histone acetylation and nucleoli abundance, while pathway analysis reveals a substantial reduction in gene expression associated with chromosome organization. In light of previous observations where the authors found a decrease in proliferation and metabolism induced by micro-topographies, they connect these findings with a quiescent phenotype in mesenchymal stem cells, as further shown by a reduction of ribosomal proteins and the maintenance of multipotency on micro-topographies after long-term culture conditions. Also, this influence of micro-topographies on nuclear morphology and proliferation is reversible, as shown by a return of proliferation when re-cultured on a flat surface. The findings provide novel insights into how biophysical signaling influences the epigenetic landscape and subsequent cellular phenotype.
Identifiants
pubmed: 36414384
doi: 10.1002/advs.202203880
pmc: PMC9811462
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2203880Subventions
Organisme : European Union's Horizon 2020 Programme
ID : H2020-MSCA-ITN-2015
Organisme : European Union's Horizon 2020 Programme
ID : 676338
Organisme : Fonds Wetenschappelijk Onderzoek
ID : 11E7920N
Organisme : Fonds Wetenschappelijk Onderzoek
ID : 11B4518N
Organisme : Fonds Wetenschappelijk Onderzoek
ID : 12E9716N
Organisme : BOF
ID : BOFDOC2021002202
Informations de copyright
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.
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