Pineapple Lectin AcmJRL Binds SARS-CoV-2 Spike Protein in a Carbohydrate-Dependent Fashion.
SARS-CoV-2
bromelain
carbohydrates
lectin
mannose
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
01 02 2023
01 02 2023
Historique:
revised:
23
11
2022
received:
10
08
2022
pubmed:
25
11
2022
medline:
4
2
2023
entrez:
24
11
2022
Statut:
ppublish
Résumé
The highly glycosylated spike protein of SARS-CoV-2 is essential for infection and constitutes a prime target for antiviral agents and vaccines. The pineapple-derived jacalin-related lectin AcmJRL is present in the medication bromelain in significant quantities and has previously been described to bind mannosides. Here, we performed a large ligand screening of AcmJRL by glycan array analysis, quantified the interaction with carbohydrates and validated high-mannose glycans as preferred ligands. Because the SARS-CoV-2 spike protein was previously reported to carry a high proportion of high-mannose N-glycans, we tested the binding of AcmJRL to the recombinantly produced extraviral domain of spike protein. We could demonstrate that AcmJRL binds the spike protein with a low-micromolar K
Identifiants
pubmed: 36420784
doi: 10.1002/cbic.202200463
pmc: PMC10107836
doi:
Substances chimiques
Carbohydrates
0
Lectins
0
Mannose
PHA4727WTP
Polysaccharides
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202200463Subventions
Organisme : National Center for Functional Glycomics (NCFG) at Beth Israel Deaconess Medical Center, Harvard Medical School
ID : R24 GM137763
Informations de copyright
© 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.
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