Dual-Wavelength Fluorescence Polarization Immunoassay for Simultaneous Detection of Sulfonamides and Antibacterial Synergists in Milk.
antibacterial synergists
dual-wavelength fluorescence polarization immunoassay
homogeneous detection
sulfonamides
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
21 Nov 2022
21 Nov 2022
Historique:
received:
24
10
2022
revised:
16
11
2022
accepted:
18
11
2022
entrez:
24
11
2022
pubmed:
25
11
2022
medline:
29
11
2022
Statut:
epublish
Résumé
Combinations of sulfonamides (SAs) and antibacterial synergists (ASGs) are frequently used for treating infectious diseases and promoting growth for animals, which cause potential hazards to food safety and human health. To realize the simultaneous detection of SAs and ASGs in food, a homogeneous and high-throughput screening dual-wavelength fluorescence polarization immunoassay (DWFPIA) was developed. In this study, three SAs tracers and three ASGs tracers were synthesized by fluoresceins with different linkers and paired with their corresponding monoclonal antibodies (mAbs), respectively. To achieve a high sensitivity and broad specificity, the combination of tracers SADMPM-HDF with the longest linker paring mAb 10E6 for SAs and tracer HaptenA-DSCA paring mAb 9C9 for ASGs were chosen for the development of DWFPIA, achieving surprising IC
Identifiants
pubmed: 36421171
pii: bios12111053
doi: 10.3390/bios12111053
pmc: PMC9688798
pii:
doi:
Substances chimiques
Sulfonamides
0
Sulfanilamide
21240MF57M
Anti-Bacterial Agents
0
Antibodies, Monoclonal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Natural Science Foundation of China
ID : 31902325
Organisme : Natural Science Foundation of Beijing Municipality
ID : 6182019
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