Novel Alligator Cathelicidin As-CATH8 Demonstrates Anti-Infective Activity against Clinically Relevant and Crocodylian Bacterial Pathogens.
LL-37
abscess model
antimicrobial peptides
biofilms
cathelicidins
reptiles
skin model
Journal
Antibiotics (Basel, Switzerland)
ISSN: 2079-6382
Titre abrégé: Antibiotics (Basel)
Pays: Switzerland
ID NLM: 101637404
Informations de publication
Date de publication:
11 Nov 2022
11 Nov 2022
Historique:
received:
28
09
2022
revised:
04
11
2022
accepted:
06
11
2022
entrez:
24
11
2022
pubmed:
25
11
2022
medline:
25
11
2022
Statut:
epublish
Résumé
Host defense peptides (HDPs) represent an alternative way to address the emergence of antibiotic resistance. Crocodylians are interesting species for the study of these molecules because of their potent immune system, which confers high resistance to infection. Profile hidden Markov models were used to screen the genomes of four crocodylian species for encoded cathelicidins and eighteen novel sequences were identified. Synthetic cathelicidins showed broad spectrum antimicrobial and antibiofilm activity against several clinically important antibiotic-resistant bacteria. In particular, the As-CATH8 cathelicidin showed potent in vitro activity profiles similar to the last-resort antibiotics vancomycin and polymyxin B. In addition, As-CATH8 demonstrated rapid killing of planktonic and biofilm cells, which correlated with its ability to cause cytoplasmic membrane depolarization and permeabilization as well as binding to DNA. As-CATH8 displayed greater antibiofilm activity than the human cathelicidin LL-37 against methicillin-resistant
Identifiants
pubmed: 36421248
pii: antibiotics11111603
doi: 10.3390/antibiotics11111603
pmc: PMC9686568
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : CIHR
ID : FDN-154287
Pays : Canada
Organisme : Consejo Nacional de Ciencia y Tecnología-Fondo Institucional de Fomento Regional para el Desarrollo Científico, Tecnológico y de Innovación (CONACyT-FORDECYT)
ID : 303045
Organisme : Dirección General de Asuntos del Personal Académico-Universidad Nacional Autónoma de México (DGAPA-UNAM)
ID : IT200321
Organisme : Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo
ID : 219RT0573
Organisme : Natural Sciences and Engineering Research Council
ID : RGPIN-2017-03831
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