Genosensing Applications of Glassy Carbon Electrodes Modified with Multi-Walled Carbon Nanotubes Non-Covalently Functionalized with Polyarginine.
DNA adsorption
carbon nanotubes
impedimetric biosensor
microRNA-21
nanotechnology
polyarginine
Journal
Micromachines
ISSN: 2072-666X
Titre abrégé: Micromachines (Basel)
Pays: Switzerland
ID NLM: 101640903
Informations de publication
Date de publication:
15 Nov 2022
15 Nov 2022
Historique:
received:
27
10
2022
revised:
02
11
2022
accepted:
11
11
2022
entrez:
24
11
2022
pubmed:
25
11
2022
medline:
25
11
2022
Statut:
epublish
Résumé
We report the advantages of glassy carbon electrodes (GCE) modified with multi-walled carbon nanotubes (MWCNTs) non-covalently functionalized with polyarginine (PolyArg) for the adsorption and electrooxidation of different DNAs and the analytical applications of the resulting platform. The presence of the carbon nanostructures, and mainly the charge of the PolyArg that supports them, facilitates the adsorption of calf-thymus and salmon sperm double-stranded DNAs and produces an important decrease in the overvoltages for the oxidation of guanine and adenine residues and a significant enhancement in the associated currents. As a proof-of-concept of possible GCE/MWCNTs-PolyArg biosensing applications, we develop an impedimetric genosensor for the quantification of microRNA-21 at femtomolar levels, using GCE/MWCNTs-PolyArg as a platform for immobilizing the DNA probe, with a detection limit of 3fM, a sensitivity of 1.544 × 10
Identifiants
pubmed: 36422406
pii: mi13111978
doi: 10.3390/mi13111978
pmc: PMC9696550
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Agencia Nacional de Promoción Científica y Tecnológica
ID : PICT 2019-04111
Organisme : FONDECYT
ID : Grant N° 1200828
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