Modulation of intestinal epithelial cell proliferation and apoptosis by Lactobacillus gasseri SF1183.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
24 11 2022
24 11 2022
Historique:
received:
24
08
2022
accepted:
16
11
2022
entrez:
24
11
2022
pubmed:
25
11
2022
medline:
29
11
2022
Statut:
epublish
Résumé
The gut microbiota exerts a variety of positive effects on the intestinal homeostasis, including the production of beneficial molecules, control of the epithelial barrier integrity and the regulation of the balance between host's cell death and proliferation. The interactions between commensal bacteria and intestinal cells are still under-investigated and is then of paramount importance to address such interactions at the molecular and cellular levels. We report an in vitro analysis of the effects of molecules secreted by Lactobacillus gasseri SF1183 on HCT116 cells, selected as a model of intestinal epithelial cells. SF1183 is a L. gasseri strain isolated from an ileal biopsy of a human healthy volunteer, able to prevent colitis symptoms in vivo. Expanding previous findings, we show that bioactive molecules secreted by SF1183 reduce the proliferation of HCT116 cells in a reversible manner determining a variation in cell cycle markers (p21WAF, p53, cyclin D1) and resulting in the protection of HCT116 cells from TNF-alfa induced apoptosis, an effect potentially relevant for the protection of the epithelial barrier integrity and reconstitution of tissue homeostasis. Consistently, SF1183 secreted molecules increase the recruitment of occludin, a major component of TJ, at the cell-cell contacts, suggesting a reinforcement of the barrier function.
Identifiants
pubmed: 36424419
doi: 10.1038/s41598-022-24483-0
pii: 10.1038/s41598-022-24483-0
pmc: PMC9691729
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
20248Informations de copyright
© 2022. The Author(s).
Références
J Appl Microbiol. 2021 Jun;130(6):1823-1838
pubmed: 33098703
Genes (Basel). 2021 Sep 29;12(10):
pubmed: 34680946
Front Microbiol. 2017 Jun 30;8:1226
pubmed: 28713353
Cell Cycle. 2007 Feb 15;6(4):407-13
pubmed: 17312393
Nutrients. 2019 Oct 14;11(10):
pubmed: 31615096
Int J Mol Sci. 2019 Apr 16;20(8):
pubmed: 30995806
Nat Rev Microbiol. 2021 Jan;19(1):55-71
pubmed: 32887946
Oncogene. 2017 Aug 24;36(34):4913-4928
pubmed: 28436949
FEMS Microbiol Rev. 2013 Nov;37(6):915-35
pubmed: 23488471
Int J Mol Sci. 2019 Sep 20;20(19):
pubmed: 31547172
J Gene Med. 2000 Sep-Oct;2(5):334-43
pubmed: 11045427
Nat Med. 2019 Jul;25(7):1096-1103
pubmed: 31263284
Front Microbiol. 2018 Aug 24;9:1785
pubmed: 30197628
Am J Physiol Gastrointest Liver Physiol. 2010 Jun;298(6):G851-9
pubmed: 20224007
Gastroenterology. 2007 Feb;132(2):562-75
pubmed: 17258729
Am J Physiol Gastrointest Liver Physiol. 2008 Nov;295(5):G1025-34
pubmed: 18787064
Bioimpacts. 2021;11(4):245-252
pubmed: 34631486
Curr Nutr Food Sci. 2013 May 1;9(2):99-107
pubmed: 24353483
BMC Microbiol. 2022 Jan 3;22(1):3
pubmed: 34979918
Cell Microbiol. 2008 Jul;10(7):1442-52
pubmed: 18331465
Gut Microbes. 2015;6(1):1-9
pubmed: 25517879
Benef Microbes. 2016 Sep;7(4):609-20
pubmed: 27291405
Cancer Res. 1994 Mar 1;54(5):1169-74
pubmed: 8118801
PLoS One. 2015 Feb 27;10(2):e0117252
pubmed: 25723571
Nat Rev Gastroenterol Hepatol. 2019 Oct;16(10):605-616
pubmed: 31296969
Gastroenterology. 2006 Mar;130(3):731-46
pubmed: 16530515
Hepatology. 2003 Feb;37(2):343-50
pubmed: 12540784
Cell Host Microbe. 2007 Jun 14;1(4):299-308
pubmed: 18005709
Sci Rep. 2019 Aug 19;9(1):12082
pubmed: 31427655
Gastroenterology. 2005 Mar;128(3):541-51
pubmed: 15765388
Front Immunol. 2021 Feb 26;12:578386
pubmed: 33717063
Pharmaceuticals (Basel). 2019 May 15;12(2):
pubmed: 31096606
Res Microbiol. 2009 Dec;160(10):817-23
pubmed: 19782749
Nutrients. 2017 May 19;9(5):
pubmed: 28534820