Comparison of reflectance confocal microscopy and line-field optical coherence tomography for the identification of keratinocyte skin tumours.


Journal

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
ISSN: 1600-0846
Titre abrégé: Skin Res Technol
Pays: England
ID NLM: 9504453

Informations de publication

Date de publication:
Jan 2023
Historique:
received: 19 05 2022
accepted: 01 10 2022
pubmed: 26 11 2022
medline: 31 1 2023
entrez: 25 11 2022
Statut: ppublish

Résumé

Reflectance confocal microscopy (RCM) and line-field confocal optical coherence tomography (LC-OCT) are non-invasive imaging devices that can help in the clinical diagnosis of actinic keratosis (AK) and cutaneous squamous cell carcinoma (SCC). No studies are available on the comparison between these two technologies for the identification of the different features of keratinocyte skin tumours. To compare RCM and LC-OCT findings in AK and SCC. A retrospective multicenter study was conducted. Tumours were imaged with RCM and LC-OCT devices before surgery, and the diagnosis was confirmed by histological examinations. LC-OCT and RCM criteria for AK/SCC were identified, and their presence/absence was evaluated in all study lesions. Gwet AC1 concordance index was calculated to compare RCM and LC-OCT. We included 52 patients with 33 AKs and 19 SCCs. Irregular epidermis was visible in most tumours and with a good degree of agreement between RCM and LC-OCT (Gwet's AC1 0.74). Parakeratosis, dyskeratotic keratinocytes and both linear dilated and glomerular vessels were better visible at LC-OCT than RCM (p < 0.001). Erosion/ulceration was identified with both methods in more than half of the cases with a good degree of agreement (Gwet AC1 0.62). Our results suggest that both LC-OCT and hand-held RCM can help clinicians in the identification of AK and SCC, providing an in vivo and non-invasive identification of an irregular epidermis. LC-OCT proved to be more effective in identifying parakeratosis, dyskeratotic keratinocytes and vessels in this series.

Sections du résumé

BACKGROUND BACKGROUND
Reflectance confocal microscopy (RCM) and line-field confocal optical coherence tomography (LC-OCT) are non-invasive imaging devices that can help in the clinical diagnosis of actinic keratosis (AK) and cutaneous squamous cell carcinoma (SCC). No studies are available on the comparison between these two technologies for the identification of the different features of keratinocyte skin tumours.
OBJECTIVES OBJECTIVE
To compare RCM and LC-OCT findings in AK and SCC.
METHODS METHODS
A retrospective multicenter study was conducted. Tumours were imaged with RCM and LC-OCT devices before surgery, and the diagnosis was confirmed by histological examinations. LC-OCT and RCM criteria for AK/SCC were identified, and their presence/absence was evaluated in all study lesions. Gwet AC1 concordance index was calculated to compare RCM and LC-OCT.
RESULTS RESULTS
We included 52 patients with 33 AKs and 19 SCCs. Irregular epidermis was visible in most tumours and with a good degree of agreement between RCM and LC-OCT (Gwet's AC1 0.74). Parakeratosis, dyskeratotic keratinocytes and both linear dilated and glomerular vessels were better visible at LC-OCT than RCM (p < 0.001). Erosion/ulceration was identified with both methods in more than half of the cases with a good degree of agreement (Gwet AC1 0.62).
CONCLUSIONS CONCLUSIONS
Our results suggest that both LC-OCT and hand-held RCM can help clinicians in the identification of AK and SCC, providing an in vivo and non-invasive identification of an irregular epidermis. LC-OCT proved to be more effective in identifying parakeratosis, dyskeratotic keratinocytes and vessels in this series.

Identifiants

pubmed: 36424847
doi: 10.1111/srt.13215
pmc: PMC9838760
doi:

Types de publication

Multicenter Study Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e13215

Informations de copyright

© 2022 The Authors. Skin Research and Technology published by John Wiley & Sons Ltd.

Références

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Auteurs

Elisa Cinotti (E)

Dermatology Unit, Department of Medical, Surgical and Neurological Sciences, Università degli Studi di Siena, Siena, Italy.
Groupe d'Imagerie non invasive de la Société Française de Dermatologie, Paris, France.

Martina Bertello (M)

Dermatology Unit, Department of Medical, Surgical and Neurological Sciences, Università degli Studi di Siena, Siena, Italy.

Alessandra Cartocci (A)

Department of Medical Biotechnologies, University of Siena, Siena, Italy.

Diletta Fiorani (D)

Dermatology Unit, Department of Medical, Surgical and Neurological Sciences, Università degli Studi di Siena, Siena, Italy.

Linda Tognetti (L)

Dermatology Unit, Department of Medical, Surgical and Neurological Sciences, Università degli Studi di Siena, Siena, Italy.

Valentina Solmi (V)

Dermatology Unit, Department of Medical, Surgical and Neurological Sciences, Università degli Studi di Siena, Siena, Italy.

Simone Cappilli (S)

Dermatology Unit, Department of Medicine and Traslational Surgery, Catholic University of Sacred Heart, Rome, Italy.
Dermatology Unit, Department of Medical and Surgical Sciences, A. Gemelli University Hospital Foundation - IRCCS, Rome, Italy.

Ketty Peris (K)

Dermatology Unit, Department of Medicine and Traslational Surgery, Catholic University of Sacred Heart, Rome, Italy.
Dermatology Unit, Department of Medical and Surgical Sciences, A. Gemelli University Hospital Foundation - IRCCS, Rome, Italy.

Jean Luc Perrot (JL)

Groupe d'Imagerie non invasive de la Société Française de Dermatologie, Paris, France.
Department of Dermatology, University Hospital of Saint-Etienne, Saint-Etienne, France.

Mariano Suppa (M)

Groupe d'Imagerie non invasive de la Société Française de Dermatologie, Paris, France.
Department of Dermatology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.
Department of Dermatology, Institut Jules Bordet, Université Libre de Bruxelles, Brussels, Belgium.

Veronique Del Marmol (V)

Department of Dermatology, Hôpital Erasme, Université Libre de Bruxelles, Brussels, Belgium.

Pietro Rubegni (P)

Dermatology Unit, Department of Medical, Surgical and Neurological Sciences, Università degli Studi di Siena, Siena, Italy.

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Classifications MeSH