Advanced quantification for single-cell adhesion by variable-angle TIRF nanoscopy.
Journal
Biophysical reports
ISSN: 2667-0747
Titre abrégé: Biophys Rep (N Y)
Pays: United States
ID NLM: 9918266001106676
Informations de publication
Date de publication:
08 Dec 2021
08 Dec 2021
Historique:
received:
26
05
2021
accepted:
07
09
2021
entrez:
25
11
2022
pubmed:
10
9
2021
medline:
10
9
2021
Statut:
epublish
Résumé
Over the last decades, several techniques have been developed to study cell adhesion; however, they present significant shortcomings. Such techniques mostly focus on strong adhesion related to specific protein-protein associations, such as ligand-receptor binding in focal adhesions. Therefore, weak adhesion, related to less specific or nonspecific cell-substrate interactions, are rarely addressed. Hence, we propose in this work a complete investigation of cell adhesion, from highly specific to nonspecific adhesiveness, using variable-angle total internal reflection fluorescence (vaTIRF) nanoscopy. This technique allows us to map in real time cell topography with a nanometric axial resolution, along with cell cortex refractive index. These two key parameters allow us to distinguish high and low adhesive cell-substrate contacts. Furthermore, vaTIRF provides cell-substrate binding energy, thus revealing a correlation between cell contractility and cell-substrate binding energy. Here, we highlight the quantitative measurements achieved by vaTIRF on U87MG glioma cells expressing different amounts of
Identifiants
pubmed: 36425460
doi: 10.1016/j.bpr.2021.100021
pii: S2667-0747(21)00021-5
pmc: PMC9680782
doi:
Types de publication
Journal Article
Langues
eng
Pagination
100021Informations de copyright
© 2021 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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