Combining fluorescence lifetime with spectral information in fluorescence lifetime imaging ophthalmoscopy (FLIO).
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Oct 2022
01 Oct 2022
Historique:
received:
08
03
2022
revised:
05
04
2022
accepted:
07
04
2022
entrez:
25
11
2022
pubmed:
26
11
2022
medline:
26
11
2022
Statut:
epublish
Résumé
Fluorescence lifetime imaging ophthalmoscopy (FLIO) provides information on fluorescence lifetimes in two spectral channels as well as the peak emission wavelength (PEW) of the fluorescence. Here, we combine these measures in an integral three-dimensional lifetime-PEW metric vector and determine a normal range for this vector from measurements in young healthy subjects. While for these control subjects 97 (±8) % (median (interquartile range)) of all para-macular pixels were covered by this normal vector range, it was 67 (±55) % for the elderly healthy, 38 (±43) % for age-related macular degeneration (AMD)-suspect subjects, and only 6 (±4) % for AMD patients. The vectors were significantly different for retinal pigment epithelium (RPE) lesions in AMD patients from that of non-affected tissue (p < 0.001). Lifetime- PEW plots allowed to identify possibly pathologic fundus areas by fluorescence parameters outside a 95% quantile per subject. In a patient follow-up, changes in fluorescence parameters could be traced in the lifetime-PEW metric, showing their change over disease progression.
Identifiants
pubmed: 36425633
doi: 10.1364/BOE.457946
pii: 457946
pmc: PMC9664887
doi:
Types de publication
Journal Article
Langues
eng
Pagination
5483-5494Informations de copyright
© 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
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