A long look at short prokaryotic Argonautes.
abortive infection
host defense
prokaryotic Argonaute (pAgo)
prokaryotic immunity
short pAgo
Journal
Trends in cell biology
ISSN: 1879-3088
Titre abrégé: Trends Cell Biol
Pays: England
ID NLM: 9200566
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
received:
05
09
2022
revised:
23
10
2022
accepted:
31
10
2022
medline:
12
6
2023
pubmed:
26
11
2022
entrez:
25
11
2022
Statut:
ppublish
Résumé
Argonaute proteins (Agos) use small 15-30 nucleotide-long guides to bind and/or cleave complementary target nucleic acids. Eukaryotic Agos mediate RNA-guided RNA silencing, while 'long' prokaryotic Agos (pAgos) use RNA or DNA guides to interfere with invading plasmid and viral DNA. Here, we review the function and mechanisms of truncated and highly divergent 'short' pAgos, which, until recently, remained functionally uncharacterized. Short pAgos have retained the Middle (MID) and P-element-Induced Wimpy Testis (PIWI) domains important for guide-mediated target binding, but lack the ability to cleave their targets. Instead, emerging insights reveal that various short pAgos interact with distinct accessory 'effector' enzymes. Upon guide-mediated detection of invading DNA by short pAgos, their associated effector enzymes kill the host cell and, consequentially, prevent spread of the invader.
Identifiants
pubmed: 36428175
pii: S0962-8924(22)00239-2
doi: 10.1016/j.tcb.2022.10.005
pii:
doi:
Substances chimiques
Argonaute Proteins
0
DNA
9007-49-2
RNA
63231-63-0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
605-618Informations de copyright
Copyright © 2022 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests D.C.S. and B.K. (together with Ana Potocnik) have submitted a patent application regarding the utilization of short pAgo systems for nucleic acid detection.