The Role of PEMFs on Bone Healing: An In Vitro Study.
bone regeneration
fracture healing
fracture repair
human adipose mesenchymal stem cells (hASCs)
human osteoblasts (hOBs)
osteogenic differentiation
pulsed electromagnetic fields (PEMFs)
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
18 Nov 2022
18 Nov 2022
Historique:
received:
20
10
2022
revised:
10
11
2022
accepted:
16
11
2022
entrez:
26
11
2022
pubmed:
27
11
2022
medline:
30
11
2022
Statut:
epublish
Résumé
Bone responses to pulsed electromagnetic fields (PEMFs) have been extensively studied by using devices that expose bone cells to PEMFs to stimulate extracellular matrix (ECM) synthesis for bone and cartilage repair. The aim of this work was to highlight in which bone healing phase PEMFs exert their action. Specifically, we evaluated the effects of PEMFs both on human adipose mesenchymal stem cells (hASCs) and on primary human osteoblasts (hOBs) by testing gene and protein expression of early bone markers (on hASCs) and the synthesis of late bone-specific proteins (on hOBs) as markers of bone remodeling. Our results indicate that PEMFs seem to exert their action on bone formation, acting on osteogenic precursors (hASCs) and inducing the commitment towards the differentiation pathways, unlike mature and terminally differentiated cells (hOBs), which are known to resist homeostasis perturbation more and seem to be much less responsive than mesenchymal stem cells. Understanding the role of PEMFs on bone regenerative processes provides important details for their clinical application.
Identifiants
pubmed: 36430775
pii: ijms232214298
doi: 10.3390/ijms232214298
pmc: PMC9693979
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Rete Apparato Muscolo Scheletrico
ID : RCR-2021-23671217
Organisme : Tesla Medical S.r.l
ID : RCR-2021-23671217
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