Phytochemicals from Vanda bensonii and Their Bioactivities to Inhibit Growth and Metastasis of Non-Small Cell Lung Cancer Cells
Vanda bensonii
anchorage-independent growth
anticancer
cell migration
cell proliferation
cytotoxicity
lung cancer
metastasis
phytochemicals
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
15 Nov 2022
15 Nov 2022
Historique:
received:
20
10
2022
revised:
04
11
2022
accepted:
14
11
2022
entrez:
26
11
2022
pubmed:
27
11
2022
medline:
30
11
2022
Statut:
epublish
Résumé
The most prevalent lung cancer is non-small cell lung cancer (NSCLC). This lung cancer type often develops other organ-specific metastases that are critical burdens in the treatment process. Orchid species in the genus Vanda have shown their potential in folkloric medication of diverse diseases but not all its species have been investigated, and little is known about their anticancer activities against NSCLC. Here, we firstly profiled the specialized metabolites of Vanda bensonii and examined their capability to inhibit growth and metastasis of NSCLC using NCI-H460 cells as a study model. Four phytochemicals, including phloretic acid methyl ester (1), cymbinodin-A (2), ephemeranthoquinone B (3), and protocatechuic acid (4), were isolated from the whole plant methanolic extract of V. bensonii. The most distinguished cytotoxic effect on NCI-H460 cells was observed in the treatments with crude methanolic extract and compound 2 with the half maximal inhibitory concentrations of 40.39 μg mL−1 and 50.82 μM, respectively. At non-cytotoxic doses (10 μg mL−1 or 10 μM), only compound 1 could significantly limit NCI-H460 cell proliferation when treated for 48 h, while others excluding compound 4 showed significant reduction in cell proliferation after treating for 72 h. Compound 1 also significantly decreased the migration rate of NCI-H460 cells examined through a wound-healing assay. Additionally, the crude extract and compound 1 strongly affected survival and growth of NCI-H460 cells under anchorage-independent conditions. Our findings proved that natural products from V. bensonii could be promising candidates for the future pharmacotherapy of NSCLC.
Identifiants
pubmed: 36432003
pii: molecules27227902
doi: 10.3390/molecules27227902
pmc: PMC9699248
pii:
doi:
Substances chimiques
Phytochemicals
0
Antineoplastic Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Thailand Science Research and Innovation Fund Chulalongkorn University
ID : CU_FRB65_hea (57)066_33_10 and CU_FRB65_hea (60)069_33_13
Références
J Asian Nat Prod Res. 2020 Jan;22(1):83-90
pubmed: 30450973
J Vis Exp. 2014 Oct 27;(92):e51998
pubmed: 25408172
Molecules. 2022 Mar 29;27(7):
pubmed: 35408617
Antioxidants (Basel). 2016 Sep 14;5(3):
pubmed: 27649250
J Ethnopharmacol. 2019 Jan 30;229:46-53
pubmed: 30268653
Cancer Treat Rev. 2014 Jul;40(6):716-22
pubmed: 24759599
Mol Clin Oncol. 2015 Jan;3(1):217-221
pubmed: 25469298
Antioxidants (Basel). 2021 Dec 31;11(1):
pubmed: 35052605
J Nat Prod. 2022 Jun 24;85(6):1591-1602
pubmed: 35679136
J Nat Prod. 2019 Jul 26;82(7):1861-1873
pubmed: 31260310
J Cell Sci. 2011 Oct 1;124(Pt 19):3189-97
pubmed: 21940791
Fitoterapia. 2011 Mar;82(2):102-40
pubmed: 20851751
J Ethnopharmacol. 2015 Nov 4;174:45-56
pubmed: 26231449
Leuk Res. 1992 Dec;16(12):1165-73
pubmed: 1361210
Heliyon. 2020 May 17;6(5):e03991
pubmed: 32455176
Nutr Cancer. 2014;66(8):1331-41
pubmed: 25356681
Anc Sci Life. 2013 Oct;33(2):92-6
pubmed: 25284941
J Nat Prod. 2012 Apr 27;75(4):605-9
pubmed: 22537363
CA Cancer J Clin. 2021 May;71(3):209-249
pubmed: 33538338
Chem Pharm Bull (Tokyo). 2012;60(9):1216-9
pubmed: 22976333
Mol Plant. 2015 Jan;8(1):40-57
pubmed: 25578271
Food Chem. 2012 May 1;132(1):482-6
pubmed: 26434319
Methods Mol Biol. 2005;294:23-9
pubmed: 15576902