Single-cell multiomics reveals the complexity of TGFβ signalling to chromatin in iPSC-derived kidney organoids.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
27 11 2022
27 11 2022
Historique:
received:
02
12
2021
accepted:
11
11
2022
pubmed:
27
11
2022
medline:
30
11
2022
entrez:
26
11
2022
Statut:
epublish
Résumé
TGFβ1 plays a regulatory role in the determination of renal cell fate and the progression of renal fibrosis. Here we show an association between SMAD3 and the histone methyltransferase, EZH2, during cell differentiation; ChIP-seq revealed that SMAD3 and EZH2 co-occupy the genome in iPSCs and in iPSC-derived nephron progenitors. Through integration of single cell gene expression and epigenome profiling, we identified de novo ACTA2
Identifiants
pubmed: 36435939
doi: 10.1038/s42003-022-04264-1
pii: 10.1038/s42003-022-04264-1
pmc: PMC9701233
doi:
Substances chimiques
Chromatin
0
Transforming Growth Factor beta
0
NUAK1 protein, human
EC 2.7.1.-
Protein Kinases
EC 2.7.-
Repressor Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1301Subventions
Organisme : NCI NIH HHS
ID : P30 CA030199
Pays : United States
Organisme : Science Foundation Ireland
ID : 19/FFP/6833
Pays : Ireland
Organisme : Science Foundation Ireland
ID : 16/IA/4584
Pays : Ireland
Informations de copyright
© 2022. The Author(s).
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