An upgraded version of carbapenem inactivation method to detect Bacteroides fragilis carbapenemase.
Bacteroides fragilis
Carbapenem inactivation method
Carbapenemase
cfiA
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Feb 2023
Feb 2023
Historique:
received:
12
07
2022
revised:
19
10
2022
accepted:
04
11
2022
pubmed:
28
11
2022
medline:
25
1
2023
entrez:
27
11
2022
Statut:
ppublish
Résumé
An increase of carbapenemase-producing Bacteroides fragilis infections is observed. To detect such a resistance in B. fragilis, several tests exist that are expensive or show poor sensitivity and specificity. Therefore, we upgraded the Anaerobic Carbapenem Inactivation Method (Ana-CIM) to easily screen for carbapenemase-producing B. fragilis. The presence of carbapenemase cfiA gene was identified in 50 B. fragilis isolates by PCR. We modified the Ana-CIM by (1) increasing the bacterial inoculum, and (2) measuring the differences in diameter between the negative control and the testing disc. We correctly classified the cfiA-negative and positive isolates and could define a cut-off of positivity at 2 mm. Our modified Ana-CIM allowed to correctly discriminate the 31 cfiA-positive with meropenem MICs ranging from 1 to > 32 µg/mL. We anticipate that our modified Ana-CIM could be used in most clinical laboratories to easily screen for carbapenemase-producing B. fragilis, even at low levels.
Identifiants
pubmed: 36436310
pii: S0732-8893(22)00221-8
doi: 10.1016/j.diagmicrobio.2022.115857
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
carbapenemase
EC 3.5.2.6
Carbapenems
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
115857Informations de copyright
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