Mass cytometry reveals cladribine-induced resets among innate lymphoid cells in multiple sclerosis.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
27 11 2022
Historique:
received: 08 07 2022
accepted: 17 11 2022
entrez: 27 11 2022
pubmed: 28 11 2022
medline: 30 11 2022
Statut: epublish

Résumé

Here we present a comprehensive mass cytometry analysis of peripheral innate lymphoid cell (ILC) subsets in relapsing/remitting MS (RRMS) patients prior to and after onset of cladribine tablets (CladT). ILC analysis was conducted on CyTOF data from peripheral blood mononuclear cells (PBMC) of MS patients before, 2 and 6 months after onset of CladT, and non-MS controls. Dimensionality reduction was used for immunophenotyping ILC subsets. CladT reduced all ILC subsets, except for CD56bright NK cells and ILC2. Furthermore, CD38+ NK cell and CCR6+ ILC3 were excluded from CladT-induced immune cell reductions. Post-CladT replenishment by immature ILC was noted by increased CD5+ ILC1 proportions at 2 months, and boosted CD38-CD56bright NK cell numbers at 6 months. CladT induce immune cell depletion among ILC but exclude CD56bright NK cells and ILC2 subsets, as well as CD38+ NK cell and CCR6+ ILC3 immunophenotypes. Post-CladT ILC expansions indicate ILC reconstitution towards a more tolerant immune system phenotype.

Identifiants

pubmed: 36437270
doi: 10.1038/s41598-022-24617-4
pii: 10.1038/s41598-022-24617-4
pmc: PMC9701791
doi:

Substances chimiques

Cladribine 47M74X9YT5

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

20411

Subventions

Organisme : Multiple Sclerosis Research Australia
ID : 17-0140
Organisme : Merck
ID : MS700568_0030

Informations de copyright

© 2022. The Author(s).

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Auteurs

F T Aglas-Leitner (FT)

Vascular Immunology Unit, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia.
Medical University of Vienna, Spitalgasse 23, Vienna, Austria.

P Juillard (P)

Vascular Immunology Unit, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia.

A Juillard (A)

Vascular Immunology Unit, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia.

S N Byrne (SN)

Centre for Immunology and Allergy Research, The Westmead Institute for Medical Research, Sydney, Australia.
Faculty of Medicine and Health, School of Medical Sciences, The University of Sydney, Sydney, Australia.

S Hawke (S)

Vascular Immunology Unit, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia.
Central West Neurology and Neurosurgery, Orange, Australia.

G E Grau (GE)

Vascular Immunology Unit, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia. georges.grau@sydney.edu.au.

F Marsh-Wakefield (F)

Vascular Immunology Unit, School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia. f.marsh-wakefield@centenary.org.au.
Liver Injury & Cancer Group, Centenary Institute, Sydney, Australia. f.marsh-wakefield@centenary.org.au.
Human Cancer & Viral Immunology Laboratory, The University of Sydney, Sydney, Australia. f.marsh-wakefield@centenary.org.au.
Centenary Institute, Sydney, NSW, Australia. f.marsh-wakefield@centenary.org.au.

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