Noncovalent Enzyme Nanogels via a Photocleavable Linkage.
Journal
Macromolecules
ISSN: 0024-9297
Titre abrégé: Macromolecules
Pays: United States
ID NLM: 0365316
Informations de publication
Date de publication:
22 Nov 2022
22 Nov 2022
Historique:
received:
29
06
2022
revised:
12
10
2022
entrez:
28
11
2022
pubmed:
29
11
2022
medline:
29
11
2022
Statut:
ppublish
Résumé
Enzyme nanogels (ENGs) offer a convenient method to protect therapeutic proteins from in vivo stressors. Current methodologies to prepare ENGs rely on either covalent modification of surface residues or the noncovalent assembly of monomers at the protein surface. In this study, we report a new method for the preparation of noncovalent ENGs that utilizes a heterobifunctional, photocleavable monomer as a hybrid approach. Initial covalent modification with this monomer established a polymerizable handle at the protein surface, followed by radical polymerization with poly(ethylene glycol) methacrylate monomer and ethylene glycol dimethacrylate crosslinker in solution. Final photoirradiation cleaved the linkage between the polymer and protein to afford the noncovalent ENGs. The enzyme phenylalanine ammonia lyase (PAL) was utilized as a model protein yielding well-defined nanogels 80 nm in size by dynamic light scattering (DLS) and 76 nm by atomic force microscopy. The stability of PAL after exposure to trypsin or low pH was assessed and was found to be more stable in the noncovalent nanogel compared to PAL alone. This approach may be useful for the stabilization of active enzymes.
Identifiants
pubmed: 36438597
doi: 10.1021/acs.macromol.2c01334
pmc: PMC9686129
doi:
Types de publication
Journal Article
Langues
eng
Pagination
9925-9933Subventions
Organisme : NIDDK NIH HHS
ID : R01 DK127908
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB013674
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008496
Pays : United States
Informations de copyright
© 2022 The Authors. Published by American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
Références
Crit Rev Biotechnol. 2018 May;38(3):335-350
pubmed: 28764566
Sci Rep. 2020 Feb 27;10(1):3651
pubmed: 32107425
ACS Cent Sci. 2018 Jun 27;4(6):718-723
pubmed: 29974067
PLoS One. 2013 Nov 15;8(11):e80581
pubmed: 24260425
EBioMedicine. 2018 Nov;37:366-373
pubmed: 30366815
Biomaterials. 2011 Aug;32(22):5223-30
pubmed: 21514660
ACS Nano. 2011 Feb 22;5(2):1385-94
pubmed: 21268592
Nano Lett. 2009 Dec;9(12):4533-8
pubmed: 19995089
Adv Mater. 2011 Oct 18;23(39):4549-53
pubmed: 21910141
Biotechnol Biotechnol Equip. 2015 Mar 4;29(2):205-220
pubmed: 26019635
Polym Chem. 2019 Jan 28;10(4):434-454
pubmed: 31249635
Proc Natl Acad Sci U S A. 1999 Mar 2;96(5):2339-44
pubmed: 10051643
J Am Chem Soc. 2018 Mar 21;140(11):4004-4017
pubmed: 29473744
Proc Natl Acad Sci U S A. 2019 Aug 13;116(33):16367-16377
pubmed: 31371509
Transl Pediatr. 2015 Oct;4(4):304-17
pubmed: 26835392
Biomacromolecules. 2007 Feb;8(2):560-5
pubmed: 17243764
Nat Nanotechnol. 2013 Mar;8(3):187-92
pubmed: 23416793
Science. 1978 Sep 1;201(4358):837-9
pubmed: 567372
J Am Chem Soc. 2019 Feb 20;141(7):2754-2769
pubmed: 30621398
Drug Deliv. 2021 Dec;28(1):1594-1602
pubmed: 34308729
Pharm Res. 2020 Feb 3;37(3):45
pubmed: 32016661
J Am Chem Soc. 2006 Aug 30;128(34):11008-9
pubmed: 16925402
Biomacromolecules. 2009 Jun 8;10(6):1612-8
pubmed: 19361213
Nat Nanotechnol. 2010 Jan;5(1):48-53
pubmed: 19935648
Mol Genet Metab. 2005 Dec;86 Suppl 1:S22-6
pubmed: 16165390
J Phys Chem B. 2008 Nov 13;112(45):14319-24
pubmed: 18939792
Bioorg Med Chem Lett. 2003 Jan 6;13(1):13-5
pubmed: 12467607
Science. 1972 Nov 24;178(4063):871-2
pubmed: 5085985
Adv Drug Deliv Rev. 2016 Apr 1;99(Pt A):28-51
pubmed: 26456916
Nat Rev Drug Discov. 2003 Mar;2(3):214-21
pubmed: 12612647
Chem Sci. 2017 Dec 1;9(4):1006-1013
pubmed: 29675147
Biomed Res Int. 2013;2013:329121
pubmed: 24106701
Science. 2009 Apr 3;324(5923):59-63
pubmed: 19342581
J Clin Invest. 1957 Oct;36(10):1521-36
pubmed: 13475490
EBioMedicine. 2018 Dec;38:15-16
pubmed: 30454929
Nat Rev Genet. 2002 Dec;3(12):954-66
pubmed: 12459725