Urinary extracellular vesicles signature for diagnosis of kidney disease.
Biomolecules
Health sciences
Nephrology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
18 Nov 2022
18 Nov 2022
Historique:
received:
30
05
2022
revised:
07
09
2022
accepted:
17
10
2022
entrez:
28
11
2022
pubmed:
29
11
2022
medline:
29
11
2022
Statut:
epublish
Résumé
Congenital disorders characterized by the quantitative and qualitative reduction in the number of functional nephrons are the primary cause of chronic kidney disease (CKD) in children. We aimed to describe the alteration of urinary extracellular vesicles (uEVs) associated with decreased renal function during childhood. By nanoparticle tracking analysis and quantitative proteomics, we identified differentially expressed proteins in uEVs in bilateral renal hypoplasia, which is characterized by a congenitally reduced number of nephrons. This expression signature of uEVs reflected decreased renal function in CKD patients by congenital anomalies of the kidney and urinary tract or ciliopathy. As a proof-of-concept, we constructed a prototype ELISA system that enabled the isolation of uEVs and quantitation of expression of molecules representing the signature. The system identified decreased renal function even in its early stage. The uEVs signature could pave the way for non-invasive methods that can complement existing testing methods for diagnosing kidney diseases.
Identifiants
pubmed: 36439984
doi: 10.1016/j.isci.2022.105416
pii: S2589-0042(22)01688-1
pmc: PMC9684058
doi:
Types de publication
Journal Article
Langues
eng
Pagination
105416Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
A patent application based on these results has been submitted by the University of Tokyo and Japanese Foundation for Cancer Research.
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