GSK3α phosphorylates dynamin-2 to promote GLUT4 endocytosis in muscle cells.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
06 02 2023
06 02 2023
Historique:
received:
19
02
2021
revised:
17
08
2022
accepted:
31
10
2022
entrez:
29
11
2022
pubmed:
30
11
2022
medline:
2
12
2022
Statut:
ppublish
Résumé
Insulin-stimulated translocation of glucose transporter 4 (GLUT4) to plasma membrane of skeletal muscle is critical for postprandial glucose uptake; however, whether the internalization of GLUT4 is also regulated by insulin signaling remains unclear. Here, we discover that the activity of dynamin-2 (Dyn2) in catalyzing GLUT4 endocytosis is negatively regulated by insulin signaling in muscle cells. Mechanistically, the fission activity of Dyn2 is inhibited by binding with the SH3 domain of Bin1. In the absence of insulin, GSK3α phosphorylates Dyn2 to relieve the inhibition of Bin1 and promotes endocytosis. Conversely, insulin signaling inactivates GSK3α and leads to attenuated GLUT4 internalization. Furthermore, the isoform-specific pharmacological inhibition of GSK3α significantly improves insulin sensitivity and glucose tolerance in diet-induced insulin-resistant mice. Together, we identify a new role of GSK3α in insulin-stimulated glucose disposal by regulating Dyn2-mediated GLUT4 endocytosis in muscle cells. These results highlight the isoform-specific function of GSK3α on membrane trafficking and its potential as a therapeutic target for metabolic disorders.
Identifiants
pubmed: 36445308
pii: 213725
doi: 10.1083/jcb.202102119
pmc: PMC9712776
pii:
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Bin1 protein, mouse
0
Dynamin II
EC 3.6.5.5
Glucose
IY9XDZ35W2
Glucose Transporter Type 4
0
Glycogen Synthase Kinase 3
EC 2.7.11.26
Insulin
0
Slc2a4 protein, mouse
0
DNM2 protein, mouse
EC 3.6.5.5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2022 Laiman et al.
Références
J Cell Sci. 2000 Nov;113 ( Pt 22):4065-76
pubmed: 11058093
Nature. 1989 Mar 2;338(6210):83-7
pubmed: 2645527
J Neuroendocrinol. 2022 Jan;34(1):e13074
pubmed: 34904299
J Clin Invest. 2008 Nov;118(11):3609-18
pubmed: 18830417
Nature. 1993 Apr 8;362(6420):557-60
pubmed: 8385271
EMBO J. 2021 Jan 15;40(2):e105513
pubmed: 33197065
Nature. 2000 Jul 6;406(6791):86-90
pubmed: 10894547
J Biol Chem. 2002 Nov 15;277(46):44108-14
pubmed: 12205083
EMBO J. 1990 Aug;9(8):2431-8
pubmed: 2164470
Science. 2002 Aug 16;297(5584):1193-6
pubmed: 12183633
Cell Metab. 2019 May 7;29(5):1119-1134.e12
pubmed: 30745182
EMBO J. 2015 Aug 13;34(16):2132-46
pubmed: 26139537
Am J Pathol. 2011 May;178(5):2224-35
pubmed: 21514436
Diabetes. 2000 Feb;49(2):263-71
pubmed: 10868943
Nat Rev Mol Cell Biol. 2011 Jul 22;12(8):517-33
pubmed: 21779028
J Biol Chem. 1997 May 16;272(20):13419-25
pubmed: 9148966
Diabetes. 2003 Mar;52(3):588-95
pubmed: 12606497
Nature. 2011 Sep 18;477(7366):556-60
pubmed: 21927000
Eur J Biochem. 1980 Jun;107(2):519-27
pubmed: 6249596
Physiol Rev. 2013 Jul;93(3):993-1017
pubmed: 23899560
Dis Model Mech. 2014 Jan;7(1):157-61
pubmed: 24135484
J Biol Chem. 2008 Dec 5;283(49):33902-10
pubmed: 18801732
Ann Intern Med. 1990 Dec 15;113(12):909-15
pubmed: 2240915
Orphanet J Rare Dis. 2010 Dec 03;5:35
pubmed: 21129173
J Clin Invest. 2017 Dec 1;127(12):4477-4487
pubmed: 29130937
J Comput Chem. 2009 May;30(7):1174-8
pubmed: 18988249
J Comput Chem. 2009 Dec;30(16):2785-91
pubmed: 19399780
Science. 2014 Oct 24;346(6208):1257998
pubmed: 25342811
Biochem Soc Trans. 2009 Oct;37(Pt 5):1022-6
pubmed: 19754444
EMBO J. 2010 Sep 15;29(18):3054-67
pubmed: 20700106
Cell Metab. 2007 Oct;6(4):329-37
pubmed: 17908561
Nat Rev Mol Cell Biol. 2012 May 23;13(6):383-96
pubmed: 22617471
Biochim Biophys Acta. 1984 Aug 14;788(3):339-47
pubmed: 6087911
Nat Genet. 2005 Nov;37(11):1207-9
pubmed: 16227997
Am J Physiol Endocrinol Metab. 2003 May;284(5):E892-900
pubmed: 12517738
Nat Rev Mol Cell Biol. 2012 Jan 11;13(2):75-88
pubmed: 22233676
Sci Transl Med. 2018 Mar 7;10(431):
pubmed: 29515000
Methods Mol Biol. 2020;2159:179-187
pubmed: 32529371
Diabetes. 2001 Aug;50(8):1901-10
pubmed: 11473054
J Alzheimers Dis. 2014;39(4):707-10
pubmed: 24254703
Sci Rep. 2017 Jul 4;7(1):4580
pubmed: 28676641
Nat Protoc. 2013 Jan;8(1):213-22
pubmed: 23288321
Nat Neurosci. 2010 Jul;13(7):845-51
pubmed: 20526333
Sci Transl Med. 2020 May 20;12(544):
pubmed: 32434848
Nat Genet. 2007 Sep;39(9):1134-9
pubmed: 17676042
Methods Enzymol. 2005;404:490-503
pubmed: 16413294
J Biol Chem. 2010 Jan 15;285(3):1653-60
pubmed: 19915010
Circ Res. 2016 Apr 15;118(8):1208-22
pubmed: 26976650
Cell. 1989 Apr 21;57(2):305-15
pubmed: 2649253
J Biol Chem. 2003 Jul 25;278(30):28312-23
pubmed: 12734182
Elife. 2017 Jun 07;6:
pubmed: 28589878
Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):983-7
pubmed: 7862678
J Am Coll Cardiol. 2014 Aug 19;64(7):696-706
pubmed: 25125302
Mol Cell Biol. 1999 Jun;19(6):4008-18
pubmed: 10330141
Cell. 2008 Dec 26;135(7):1263-75
pubmed: 19084268
J Biol Chem. 2010 Jul 23;285(30):22753-7
pubmed: 20529869
Brain. 2014 Dec;137(Pt 12):3160-70
pubmed: 25260562
Trends Pharmacol Sci. 2004 Sep;25(9):471-80
pubmed: 15559249
Sci Transl Med. 2019 Mar 20;11(484):
pubmed: 30894500
Nat Commun. 2017 Jun 07;8:15661
pubmed: 28589938
Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9660-4
pubmed: 9275179
J Clin Invest. 2014 Mar;124(3):1350-63
pubmed: 24569376
J Biol Chem. 1996 Dec 6;271(49):31372-8
pubmed: 8940145
Neurotherapeutics. 2018 Oct;15(4):966-975
pubmed: 30426359
Cell Metab. 2020 Nov 3;32(5):844-859.e5
pubmed: 32888406
J Neurosci. 2009 Jun 17;29(24):7706-17
pubmed: 19535582
Mol Cell. 2009 Oct 23;36(2):326-39
pubmed: 19854140
J Mol Biol. 1996 Jan 12;255(1):14-21
pubmed: 8568861
J Diabetes Sci Technol. 2008 Nov;2(6):1101-13
pubmed: 19885299
Genes Dev. 2001 Nov 15;15(22):2967-79
pubmed: 11711432
Proc Natl Acad Sci U S A. 2022 Mar 1;119(9):
pubmed: 35217605
Hum Mol Genet. 2015 Oct 1;24(19):5542-54
pubmed: 26199319
Mol Ther. 2022 Feb 2;30(2):868-880
pubmed: 34371181
ACS Chem Biol. 2016 Jul 15;11(7):1952-63
pubmed: 27128528
Biochem J. 1994 Nov 1;303 ( Pt 3):701-4
pubmed: 7980435
Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):E234-42
pubmed: 21670293
Skelet Muscle. 2011 Jul 13;1(1):26
pubmed: 21797990
Nature. 2010 May 27;465(7297):435-40
pubmed: 20428113
Nature. 1995 Dec 21-28;378(6559):785-9
pubmed: 8524413
Proc Natl Acad Sci U S A. 1989 Apr;86(8):2535-9
pubmed: 2649883
Science. 1998 Aug 7;281(5378):821-4
pubmed: 9694653
Mol Cell. 2001 Jun;7(6):1321-7
pubmed: 11430833
Am J Physiol. 1985 Mar;248(3 Pt 1):E353-62
pubmed: 3883806