Sample Preparation for Mass Spectrometry-Based Absolute Quantification of Bacterial Proteins in Antibiotic Stress Research.

Bacterial Proteins Anti-Bacterial Agents / pharmacology Mass Spectrometry Specimen Handling Membrane Proteins
Absolute protein quantification Antibiotic stress response Cytosol Gel free proteomics In-solution digest Mass spectrometry Membrane S-trap Sample preparation

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2023
Historique:
entrez: 29 11 2022
pubmed: 30 11 2022
medline: 2 12 2022
Statut: ppublish

Résumé

Absolute protein quantification is an essential tool for system biology approaches and elucidation of stoichiometry of multi-protein complexes. In this updated chapter, a universal protocol for gel-free absolute protein quantification in bacterial systems is described, which provides adapted methods for cytosolic and membrane proteins. This protocol can be used for sample preparation prior to miscellaneous mass spectrometry-based quantification workflows like AQUA, Hi3, and emPAI. In addition, a focus has been set to the specific challenges in antibiotic stress research.

Identifiants

DOI: 10.1007/978-1-0716-2855-3_18 PMID: 36445593
pubmed: 36445593
doi: 10.1007/978-1-0716-2855-3_18
doi:

Substances chimiques

Bacterial Proteins 0
Anti-Bacterial Agents 0
Membrane Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

335-348

Informations de copyright

© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

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doi: 10.1016/j.jprot.2016.01.015
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doi: 10.1007/978-1-4939-6634-9_17

Auteurs

Sandra Maaß (S)

Department of Microbial Proteomics, University of Greifswald, Institute of Microbiology, Greifswald, Germany. sandra.maass@uni-greifswald.de.

Minia Antelo-Varela (M)

Department of Microbial Proteomics, University of Greifswald, Institute of Microbiology, Greifswald, Germany.
University of Basel, Biozentrum, Focal Area Infection Biology, Basel, Switzerland.

Florian Bonn (F)

Department of Microbial Proteomics, University of Greifswald, Institute of Microbiology, Greifswald, Germany.
Immundiagnostik AG, Bensheim, Germany.

Dörte Becher (D)

Department of Microbial Proteomics, University of Greifswald, Institute of Microbiology, Greifswald, Germany.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Sample Preparation for Mass Spectrometry-Based...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Sample Preparation for Mass Spectrometry-Based...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Sample Preparation for Mass Spectrometry-Based...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Manipulation d'échantillons › Sample Preparation for Mass Spectrometry-Based...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Sample Preparation for Mass Spectrometry-Based...