In vitro evaluation of gentamicin activity against Spanish field isolates of Brachyspira hyodysenteriae.
Aminoglycoside
Antibiotic
Antimicrobial susceptibility testing
Resistance
Spirochaetes
Swine dysentery
Journal
Porcine health management
ISSN: 2055-5660
Titre abrégé: Porcine Health Manag
Pays: England
ID NLM: 101684126
Informations de publication
Date de publication:
01 Dec 2022
01 Dec 2022
Historique:
received:
01
07
2022
accepted:
14
10
2022
entrez:
1
12
2022
pubmed:
2
12
2022
medline:
2
12
2022
Statut:
epublish
Résumé
The treatment of swine dysentery (SD) has become constrained in recent years due to the limited availability of effective drugs combined with a rise in antimicrobial resistance. Gentamicin, an aminoglycoside antibiotic, is authorised for the control of this disease in several European countries but has not been extensively used so far. In this study, the in vitro susceptibility of 56 Brachyspira hyodysenteriae field isolates was evaluated against gentamicin using a broth microdilution test. The molecular basis of decreased susceptibility to gentamicin was also investigated by sequencing the 16S rRNA gene and phylogenetic relatedness by multiple-locus variable number tandem-repeat analysis (MLVA). Most B. hyodysenteriae isolates presented low minimum inhibitory concentration (MIC) values to gentamicin, with a mode of 2 µg/mL, a median or MIC The results provide an accurate characterisation of antimicrobial sensitivity to gentamicin and possible mechanisms of resistance in Spanish B. hyodysenteriae isolates. These findings allow us to propose gentamicin as an alternative in the antibiotic management of SD, particularly in outbreaks caused by pleuromutilin resistant isolates.
Sections du résumé
BACKGROUND
BACKGROUND
The treatment of swine dysentery (SD) has become constrained in recent years due to the limited availability of effective drugs combined with a rise in antimicrobial resistance. Gentamicin, an aminoglycoside antibiotic, is authorised for the control of this disease in several European countries but has not been extensively used so far. In this study, the in vitro susceptibility of 56 Brachyspira hyodysenteriae field isolates was evaluated against gentamicin using a broth microdilution test. The molecular basis of decreased susceptibility to gentamicin was also investigated by sequencing the 16S rRNA gene and phylogenetic relatedness by multiple-locus variable number tandem-repeat analysis (MLVA).
RESULTS
RESULTS
Most B. hyodysenteriae isolates presented low minimum inhibitory concentration (MIC) values to gentamicin, with a mode of 2 µg/mL, a median or MIC
CONCLUSIONS
CONCLUSIONS
The results provide an accurate characterisation of antimicrobial sensitivity to gentamicin and possible mechanisms of resistance in Spanish B. hyodysenteriae isolates. These findings allow us to propose gentamicin as an alternative in the antibiotic management of SD, particularly in outbreaks caused by pleuromutilin resistant isolates.
Identifiants
pubmed: 36451249
doi: 10.1186/s40813-022-00291-w
pii: 10.1186/s40813-022-00291-w
pmc: PMC9714239
doi:
Types de publication
Journal Article
Langues
eng
Pagination
48Subventions
Organisme : Junta de Castilla y León
ID : ULE-02-B
Organisme : MInisterio de ciencia e innovacion, Spain
ID : PRE2020-093762
Organisme : Ministerio de Universidades, Spain
ID : BEAGAL-18-106
Organisme : Consejería de Educación, Junta de Castilla y León
ID : LE131-18
Organisme : Ministerio de Educación y Formación Profesional
ID : FPU17/00466
Informations de copyright
© 2022. The Author(s).
Références
Medchemcomm. 2016 Jan 1;7(1):11-27
pubmed: 26877861
Microbiol Spectr. 2020 Jan;8(1):
pubmed: 31971162
Vet Microbiol. 2002 Jan 3;84(1-2):123-33
pubmed: 11731165
Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):9716-21
pubmed: 23630274
J Vet Pharmacol Ther. 1991 Mar;14(1):1-35
pubmed: 2038090
Antimicrob Agents Chemother. 2011 Jul;55(7):3330-7
pubmed: 21555771
Infect Dis Clin North Am. 2016 Jun;30(2):523-537
pubmed: 27208771
Res Vet Sci. 2015 Dec;103:51-3
pubmed: 26679795
J Clin Microbiol. 2016 Aug;54(8):2109-19
pubmed: 27252458
J Vet Diagn Invest. 1998 Oct;10(4):350-6
pubmed: 9786523
Pol J Vet Sci. 2012;15(2):259-65
pubmed: 22844703
Microb Drug Resist. 2018 Jun;24(5):685-692
pubmed: 29653474
FEMS Microbiol Lett. 1999 Mar 15;172(2):255-60
pubmed: 10188254
Vet Microbiol. 2007 Jul 20;123(1-3):245-8
pubmed: 17428623
Vet Microbiol. 2020 May;244:108645
pubmed: 32402332
Vet Pathol. 2017 Jan;54(1):22-31
pubmed: 27288432
Front Microbiol. 2018 Jun 19;9:1183
pubmed: 29971045
J Vet Diagn Invest. 2011 Nov;23(6):1140-5
pubmed: 22362794
Antimicrob Agents Chemother. 2005 Aug;49(8):3192-7
pubmed: 16048924
Vet Microbiol. 2014 Aug 6;172(1-2):223-9
pubmed: 24948419
Prev Vet Med. 2006 Apr 17;74(1):56-66
pubmed: 16488031
Acta Vet Scand. 2012 Sep 21;54:54
pubmed: 22998753
Microb Drug Resist. 2011 Sep;17(3):485-8
pubmed: 21663534
Can J Microbiol. 2017 Jun;63(6):465-474
pubmed: 28324657
J Clin Microbiol. 2010 Aug;48(8):2859-65
pubmed: 20554811
Antimicrob Agents Chemother. 2001 Sep;45(9):2414-9
pubmed: 11502507
J Vet Med Sci. 2010 Dec;72(12):1651-3
pubmed: 20697185
Int J Environ Res Public Health. 2013 May 10;10(5):1927-47
pubmed: 23665849
Vet Microbiol. 2019 Feb;229:59-71
pubmed: 30642599
Vet Microbiol. 2004 Aug 19;102(1-2):25-32
pubmed: 15288924
Microbiol Rev. 1987 Dec;51(4):439-57
pubmed: 3325794
Nucleic Acids Res. 2019 Feb 20;47(3):1557-1563
pubmed: 30476222
Schweiz Arch Tierheilkd. 2016 Jun;158(6):405-10
pubmed: 27504836
Med Clin North Am. 1970 Sep;54(5):1305-15
pubmed: 4919154
Vet Microbiol. 2017 Aug;207:125-132
pubmed: 28757011
Mol Biol Evol. 2021 Jun 25;38(7):3022-3027
pubmed: 33892491
PLoS One. 2012;7(3):e33275
pubmed: 22479378
Mol Microbiol. 2004 Dec;54(5):1295-306
pubmed: 15554969
PLoS One. 2016 Dec 1;11(12):e0167424
pubmed: 27907102
Vet J. 2015 Jan;203(1):115-9
pubmed: 25486860
J Clin Microbiol. 2003 Jun;41(6):2596-604
pubmed: 12791886