High-efficiency retron-mediated single-stranded DNA production in plants.
CRISPR-Cas9
HDR
VirE2
retron
ssDNA
Journal
Synthetic biology (Oxford, England)
ISSN: 2397-7000
Titre abrégé: Synth Biol (Oxf)
Pays: England
ID NLM: 101728857
Informations de publication
Date de publication:
2022
2022
Historique:
received:
06
09
2022
revised:
20
10
2022
accepted:
30
10
2022
entrez:
1
12
2022
pubmed:
2
12
2022
medline:
2
12
2022
Statut:
epublish
Résumé
Retrons are a class of retroelements that produce multicopy single-stranded DNA (ssDNA) and participate in anti-phage defenses in bacteria. Retrons have been harnessed for the overproduction of ssDNA, genome engineering and directed evolution in bacteria, yeast and mammalian cells. Retron-mediated ssDNA production in plants could unlock their potential applications in plant biotechnology. For example, ssDNA can be used as a template for homology-directed repair (HDR) in several organisms. However, current gene editing technologies rely on the physical delivery of synthetic ssDNA, which limits their applications. Here, we demonstrated retron-mediated overproduction of ssDNA in
Identifiants
pubmed: 36452068
doi: 10.1093/synbio/ysac025
pii: ysac025
pmc: PMC9700382
doi:
Types de publication
Journal Article
Langues
eng
Pagination
ysac025Informations de copyright
© The Author(s) 2022. Published by Oxford University Press.
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