Haemophilus ducreyi Infection Induces Oxidative Stress, Central Metabolic Changes, and a Mixed Pro- and Anti-inflammatory Environment in the Human Host.
Haemophilus ducreyi
dual RNA-seq
human
interactome
metabolome
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
20 12 2022
20 12 2022
Historique:
entrez:
1
12
2022
pubmed:
2
12
2022
medline:
3
3
2023
Statut:
ppublish
Résumé
Few studies have investigated host-bacterial interactions at sites of infection in humans using transcriptomics and metabolomics. Haemophilus ducreyi causes cutaneous ulcers in children and the genital ulcer disease chancroid in adults. We developed a human challenge model in which healthy adult volunteers are infected with H. ducreyi on the upper arm until they develop pustules. Here, we characterized host-pathogen interactions in pustules using transcriptomics and metabolomics and examined interactions between the host transcriptome and metabolome using integrated omics. In a previous pilot study, we determined the human and H. ducreyi transcriptomes and the metabolome of pustule and wounded sites of 4 volunteers (B. Griesenauer, T. M. Tran, K. R. Fortney, D. M. Janowicz, et al., mBio 10:e01193-19, 2019, https://doi.org/10.1128/mBio.01193-19). While we could form provisional transcriptional networks between the host and H. ducreyi, the study was underpowered to integrate the metabolome with the host transcriptome. To better define and integrate the transcriptomes and metabolome, we used samples from both the pilot study (
Identifiants
pubmed: 36453940
doi: 10.1128/mbio.03125-22
pmc: PMC9765465
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0312522Subventions
Organisme : NIAID NIH HHS
ID : R01 AI137116
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007637
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002529
Pays : United States
Commentaires et corrections
Type : ErratumIn
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