The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
01 12 2022
01 12 2022
Historique:
received:
03
09
2021
accepted:
09
11
2022
entrez:
1
12
2022
pubmed:
2
12
2022
medline:
6
12
2022
Statut:
epublish
Résumé
Fluorescence laser-scanning microscopy (LSM) is experiencing a revolution thanks to new single-photon (SP) array detectors, which give access to an entirely new set of single-photon information. Together with the blooming of new SP LSM techniques and the development of tailored SP array detectors, there is a growing need for (i) DAQ systems capable of handling the high-throughput and high-resolution photon information generated by these detectors, and (ii) incorporating these DAQ protocols in existing fluorescence LSMs. We developed an open-source, low-cost, multi-channel time-tagging module (TTM) based on a field-programmable gate array that can tag in parallel multiple single-photon events, with 30 ps precision, and multiple synchronisation events, with 4 ns precision. We use the TTM to demonstrate live-cell super-resolved fluorescence lifetime image scanning microscopy and fluorescence lifetime fluctuation spectroscopy. We expect that our BrightEyes-TTM will support the microscopy community in spreading SP-LSM in many life science laboratories.
Identifiants
pubmed: 36456575
doi: 10.1038/s41467-022-35064-0
pii: 10.1038/s41467-022-35064-0
pmc: PMC9715684
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7406Informations de copyright
© 2022. The Author(s).
Références
Opt Express. 2018 Aug 20;26(17):22234-22248
pubmed: 30130919
Rev Sci Instrum. 2017 May;88(5):053701
pubmed: 28571439
Opt Express. 2003 Dec 29;11(26):3583-91
pubmed: 19471494
Biophys J. 2005 Dec;89(6):4029-42
pubmed: 16199500
Nat Commun. 2022 Dec 1;13(1):7406
pubmed: 36456575
Phys Rev Lett. 2010 May 14;104(19):198101
pubmed: 20867000
Biophys J. 2008 Feb 15;94(4):1437-48
pubmed: 17933881
Biophys J. 2005 May;88(5):3584-91
pubmed: 15695633
IEEE Rev Biomed Eng. 2023;16:627-652
pubmed: 34166201
Opt Express. 2011 Dec 5;19(25):25006-21
pubmed: 22273893
Light Sci Appl. 2021 Feb 5;10(1):31
pubmed: 33542179
Nat Commun. 2019 Jul 15;10(1):3103
pubmed: 31308370
Biomed Opt Express. 2020 May 07;11(6):2905-2924
pubmed: 32637232
J Microsc. 2008 Jan;229(Pt 1):78-91
pubmed: 18173647
PLoS One. 2020 Dec 30;15(12):e0238327
pubmed: 33378370
Biophys J. 2011 Jun 8;100(11):2839-45
pubmed: 21641330
Nat Commun. 2018 Nov 30;9(1):5120
pubmed: 30504919
Biophys J. 2008 Jan 15;94(2):L14-6
pubmed: 17981902
Cell Rep. 2017 Mar 14;18(11):2795-2806
pubmed: 28297680
Light Sci Appl. 2019 Sep 18;8:87
pubmed: 31645931
Nat Methods. 2018 Sep;15(9):669-676
pubmed: 30171252
Nat Methods. 2021 May;18(5):542-550
pubmed: 33859440
Nat Methods. 2019 Feb;16(2):175-178
pubmed: 30643212
Biophys Rep (N Y). 2021 Dec 08;1(2):None
pubmed: 34939046
Nat Protoc. 2018 Sep;13(9):1979-2004
pubmed: 30190551
Neuron. 2017 Jul 5;95(1):63-69.e5
pubmed: 28683270
IEEE J Sel Top Quantum Electron. 2019 Jan-Feb;25(1):
pubmed: 31156324
Nature. 2017 Nov 30;551(7682):659-662
pubmed: 29189791
Appl Spectrosc. 2014;68(5):577-83
pubmed: 25014602
Nat Nanotechnol. 2019 Oct;14(10):981-987
pubmed: 31527841
Nat Rev Methods Primers. 2021;1:
pubmed: 35663461
Opt Express. 2019 Nov 11;27(23):32863-32882
pubmed: 31878363
Nat Commun. 2014 Jun 04;5:3921
pubmed: 24894704
Int J Mol Sci. 2012 Oct 09;13(10):12890-910
pubmed: 23202928
Curr Opin Biotechnol. 2005 Feb;16(1):19-27
pubmed: 15722011
Biophys J. 2006 Jun 1;90(11):L80-2
pubmed: 16617080
Anal Biochem. 2007 Sep 1;368(1):95-9
pubmed: 17586454