CRISPR-Cas9: Taming protozoan parasites with bacterial scissor.
CRISPR
Entamoeba
Leishmania
Plasmodium
Protozoa
Toxoplasma
Trichomonas
Trypanosoma
Journal
Journal of parasitic diseases : official organ of the Indian Society for Parasitology
ISSN: 0971-7196
Titre abrégé: J Parasit Dis
Pays: India
ID NLM: 9713059
Informations de publication
Date de publication:
Dec 2022
Dec 2022
Historique:
received:
30
05
2022
accepted:
12
09
2022
entrez:
2
12
2022
pubmed:
3
12
2022
medline:
3
12
2022
Statut:
ppublish
Résumé
The invention of CRISPR-Cas9 technology has opened a new era in which genome manipulation has become precise, faster, cheap and more accurate than previous genome editing strategies. Despite the intricacies of the genomes associated with several protozoan parasites, CRISPR-Cas9 has made a substantial contribution to parasitology. The study of functional genomics through CRISPR-Cas9 mediated gene knockout, insertion, deletion and mutation has helped in understanding intrinsic parasite biology. The invention of CRISPR-dCas9 has helped in the programmable control of protozoan gene expression and epigenetic engineering. CRISPR and CRISPR-based alternatives will continue to thrive and may aid in the development of novel anti-protozoan strategies to tame the protozoan parasites in the imminent future.
Identifiants
pubmed: 36457766
doi: 10.1007/s12639-022-01534-x
pii: 1534
pmc: PMC9606157
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
1204-1212Informations de copyright
© Indian Society for Parasitology 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
Déclaration de conflit d'intérêts
Conflict of interestNot applicable.
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