Development and validation of a novel chiral chromatographic method for separation of lenalidomide enantiomers in human plasma.
CSP-HPLC
chirality
enantiomers
lenalidomide
validation
Journal
Chirality
ISSN: 1520-636X
Titre abrégé: Chirality
Pays: United States
ID NLM: 8914261
Informations de publication
Date de publication:
02 2023
02 2023
Historique:
revised:
05
11
2022
received:
12
04
2022
accepted:
11
11
2022
pubmed:
3
12
2022
medline:
21
1
2023
entrez:
2
12
2022
Statut:
ppublish
Résumé
Lenalidomide (LND) is an analogue of thalidomide that is second generation immunomodulatory drugs (IMiDs). LND contains asymmetric carbon atom and exist R and S enantiomer. S (-) form of enantiomer are considered to be more potent and biologically active in tumor cell. It is available in racemic form for clinical use. The study aims to develop and validate enantiomer separation of LND in human plasma. The chromatographic enantiomeric separation was achieved on a Daicel-CSP, Chiralpack IA 4.6 × 250 mm_5 μm. The mobile phase was constituted in combination of methanol:glacial acetic acid at a concentration of 499.50 ml: 50 μl. UV wavelength detection was 220 nm. The RSD% for all validation parameters was found to be within the acceptable limit. The chiral chromatographic (chiral stationary phase-high-performance liquid chromatography [CSP-HPLC]) method developed and validated for the quantitative estimation of LND enantiomers S (-) and R (+) in human plasma sample is accurate, precise, robust, stable and selective.
Substances chimiques
Lenalidomide
F0P408N6V4
Thalidomide
4Z8R6ORS6L
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
83-91Informations de copyright
© 2022 Wiley Periodicals LLC.
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