Extending resolution within a single imaging frame.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
02 12 2022
02 12 2022
Historique:
received:
22
10
2021
accepted:
27
10
2022
entrez:
2
12
2022
pubmed:
3
12
2022
medline:
7
12
2022
Statut:
epublish
Résumé
The resolution of fluorescence microscopy images is limited by the physical properties of light. In the last decade, numerous super-resolution microscopy (SRM) approaches have been proposed to deal with such hindrance. Here we present Mean-Shift Super Resolution (MSSR), a new SRM algorithm based on the Mean Shift theory, which extends spatial resolution of single fluorescence images beyond the diffraction limit of light. MSSR works on low and high fluorophore densities, is not limited by the architecture of the optical setup and is applicable to single images as well as temporal series. The theoretical limit of spatial resolution, based on optimized real-world imaging conditions and analysis of temporal image stacks, has been measured to be 40 nm. Furthermore, MSSR has denoising capabilities that outperform other SRM approaches. Along with its wide accessibility, MSSR is a powerful, flexible, and generic tool for multidimensional and live cell imaging applications.
Identifiants
pubmed: 36460648
doi: 10.1038/s41467-022-34693-9
pii: 10.1038/s41467-022-34693-9
pmc: PMC9718789
doi:
Substances chimiques
Drugs, Generic
0
Fluorescent Dyes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7452Informations de copyright
© 2022. The Author(s).
Références
Opt Lett. 1994 Jun 1;19(11):780-2
pubmed: 19844443
Nat Commun. 2021 Mar 19;12(1):1748
pubmed: 33741958
Biophys Rep (N Y). 2021 Sep 8;1(1):
pubmed: 35382035
Epigenetics. 2012 Jul;7(7):747-57
pubmed: 22647320
Curr Protoc Mol Biol. 2010 Oct;Chapter 14:Unit14.20
pubmed: 20890901
J Cell Sci. 2020 Jun 11;133(11):
pubmed: 32527967
Nano Lett. 2021 Apr 14;21(7):2752-2757
pubmed: 33729813
J Cell Sci. 2011 May 15;124(Pt 10):1607-11
pubmed: 21536831
Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):4797-8
pubmed: 16549771
Methods. 2017 Feb 15;115:80-90
pubmed: 27713081
Nat Methods. 2018 Sep;15(9):689-692
pubmed: 30061677
Biomed Eng Online. 2015 Mar 20;14:24
pubmed: 25884695
Opt Express. 2020 Nov 9;28(23):34434-34449
pubmed: 33182913
Opt Express. 2021 Jul 19;29(15):23368-23380
pubmed: 34614603
Nat Methods. 2015 Jun;12(6):503-13
pubmed: 26020503
Nat Methods. 2013 Jul;10(7):653-8
pubmed: 23708387
Genome Biol. 2019 Aug 7;20(1):157
pubmed: 31391082
Science. 1995 Jun 9;268(5216):1483-7
pubmed: 7770772
Exp Mol Med. 2018 Aug 28;50(8):1-12
pubmed: 30154456
Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63
pubmed: 20150100
Nat Methods. 2014 Mar;11(3):281-9
pubmed: 24441936
Plant J. 2009 Jul;59(1):169-78
pubmed: 19309456
Nat Methods. 2019 Sep;16(9):853-857
pubmed: 31427757
J Phys D Appl Phys. 2019 Apr 17;52(16):163001
pubmed: 33191949
Opt Express. 2014 Apr 21;22(8):9159-70
pubmed: 24787806
J Microsc. 2018 Sep;271(3):266-281
pubmed: 29797718
Angew Chem Int Ed Engl. 2015 Jul 6;54(28):8034-53
pubmed: 26087684
Nat Methods. 2015 Aug;12(8):717-24
pubmed: 26076424
Nat Methods. 2017 Jul 28;14(8):760-761
pubmed: 28753600
Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22287-92
pubmed: 20018714
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Sci Rep. 2016 Jun 06;6:27290
pubmed: 27264341
Proc Natl Acad Sci U S A. 2017 Aug 15;114(33):E6857-E6866
pubmed: 28760978
Sci Rep. 2018 Apr 3;8(1):5581
pubmed: 29615650
J Biomed Opt. 2015 Oct;20(10):101206
pubmed: 26527322
Bioinformatics. 2015 Apr 15;31(8):1279-85
pubmed: 25480371
Mol Reprod Dev. 2020 Dec;87(12):1188-1198
pubmed: 33118273
Nat Protoc. 2017 Jun;12(6):1198-1228
pubmed: 28518172
Nat Commun. 2016 Dec 09;7:13752
pubmed: 27934858
Elife. 2019 Jul 25;8:
pubmed: 31343403
J Cell Sci. 2018 Nov 8;131(21):
pubmed: 30301778
Sci Rep. 2015 Dec 08;5:17740
pubmed: 26643905
Nat Methods. 2019 May;16(5):387-395
pubmed: 30962624
Biophys J. 2007 Aug 1;93(3):1079-88
pubmed: 17483157
Nat Methods. 2013 Jul;10(7):598-9
pubmed: 23749304
Philos Trans A Math Phys Eng Sci. 2022 Apr 4;380(2220):20210109
pubmed: 35152757
Nat Methods. 2018 Apr;15(4):263-266
pubmed: 29457791
IEEE Trans Image Process. 2003;12(8):857-65
pubmed: 18237960
Commun Biol. 2020 Aug 20;3(1):458
pubmed: 32820217
Chemphyschem. 2014 Mar 17;15(4):577-86
pubmed: 24615819
Nat Methods. 2019 Nov;16(11):1101-1104
pubmed: 31591576
Biophys J. 2001 Oct;81(4):2378-88
pubmed: 11566807
Magn Reson Med. 1996 Dec;36(6):923-31
pubmed: 8946358
Proc Natl Acad Sci U S A. 2020 Aug 25;117(34):20943-20949
pubmed: 32817465
Science. 2006 Sep 15;313(5793):1642-5
pubmed: 16902090
Cell. 2015 Mar 12;160(6):1145-58
pubmed: 25768910
Nat Methods. 2012 Apr 01;9(5):483-5
pubmed: 22466793
Opt Express. 2012 Sep 24;20(20):22585-601
pubmed: 23037408
Methods Mol Biol. 2021;2304:111-130
pubmed: 34028713
PLoS One. 2015 Nov 04;10(11):e0142277
pubmed: 26535902
Nat Commun. 2020 Jan 3;11(1):94
pubmed: 31901080
Chromosoma. 2016 Jun;125(2):189-203
pubmed: 26440409
Opt Express. 2018 Aug 20;26(17):21887-21899
pubmed: 30130891
Biomed Opt Express. 2013 Oct 25;4(11):2644-56
pubmed: 24298422
Biophys J. 2011 Dec 21;101(12):2855-70
pubmed: 22208184
Nat Methods. 2008 Aug;5(8):695-702
pubmed: 18641657
Nat Methods. 2011 Dec 04;9(2):195-200
pubmed: 22138825
Nat Commun. 2016 Aug 12;7:12471
pubmed: 27514992
Philos Trans A Math Phys Eng Sci. 2021 Jun 14;379(2199):20200143
pubmed: 33896205
Nat Methods. 2019 Sep;16(9):918-924
pubmed: 31451766