Stiffness of primordial germ cells is required for their extravasation in avian embryos.
Biological sciences
Cell biology
Developmental biology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
22 Dec 2022
22 Dec 2022
Historique:
received:
11
07
2022
revised:
14
10
2022
accepted:
16
11
2022
entrez:
5
12
2022
pubmed:
6
12
2022
medline:
6
12
2022
Statut:
epublish
Résumé
Unlike mammals, primordial germ cells (PGCs) in avian early embryos exploit blood circulation to translocate to the somatic gonadal primordium, but how circulating PGCs undergo extravasation remains elusive. We demonstrate with single-cell level live-imaging analyses that the PGCs are arrested at a specific site in the capillary plexus, which is predominantly governed by occlusion at a narrow path in the vasculature. The occlusion is enabled by a heightened stiffness of the PGCs mediated by actin polymerization. Following the occlusion, PGCs reset their stiffness to soften in order to squeeze through the endothelial lining as they transmigrate. Our discovery also provides a model for the understanding of metastasizing cancer extravasation occurring mainly by occlusion.
Identifiants
pubmed: 36465120
doi: 10.1016/j.isci.2022.105629
pii: S2589-0042(22)01901-0
pmc: PMC9713369
doi:
Types de publication
Journal Article
Langues
eng
Pagination
105629Informations de copyright
© 2022 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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