Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants.

severe acute respiratory syndrome coronavirus 2 subgenomic RNA variants of concern

Journal

Open forum infectious diseases
ISSN: 2328-8957
Titre abrégé: Open Forum Infect Dis
Pays: United States
ID NLM: 101637045

Informations de publication

Date de publication:
Nov 2022
Historique:
received: 08 09 2022
accepted: 08 11 2022
entrez: 5 12 2022
pubmed: 6 12 2022
medline: 6 12 2022
Statut: epublish

Résumé

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) subgenomic RNA (sgRNA) may indicate actively replicating virus, but sgRNA abundance has not been systematically compared between SARS-CoV-2 variants. sgRNA was quantified in 169 clinical samples by real-time reverse-transcription polymerase chain reaction, demonstrating similar relative abundance among known variants. Thus, sgRNA detection can identify individuals with active viral replication regardless of variant.

Identifiants

pubmed: 36467291
doi: 10.1093/ofid/ofac619
pii: ofac619
pmc: PMC9709700
doi:

Types de publication

Journal Article

Langues

eng

Pagination

ofac619

Subventions

Organisme : NCATS NIH HHS
ID : UL1 TR002378
Pays : United States

Informations de copyright

© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America.

Déclaration de conflit d'intérêts

Potential conflicts of interest. All authors: No reported conflicts of interest.

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Auteurs

Maxwell Su (M)

Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, Georgia, USA.

Sara Ping (S)

Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, Georgia, USA.

Phuong-Vi Nguyen (PV)

Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, Georgia, USA.

Alejandra Rojas (A)

Departamento de Producción, Universidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud, Asunción, Paraguay.

Laila Hussaini (L)

Department of Pediatrics and Children's Healthcare of Atlanta, Emory University, Atlanta, Georgia, USA.

Ludy Registre Carmola (LR)

Department of Pathology, Emory University, Atlanta, Georgia, USA.

Azmain Taz (A)

Department of Pathology, Emory University, Atlanta, Georgia, USA.

Julie Sullivan (J)

Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.

Greg S Martin (GS)

Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.
Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine, Emory University, Atlanta, Georgia, USA.

Anne Piantadosi (A)

Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, Georgia, USA.
Department of Pathology, Emory University, Atlanta, Georgia, USA.
Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.

Magaly Martinez (M)

Departamento de Biología Molecular y Biotecnología, Universidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud, Asunción, Paraguay.

Wilbur A Lam (WA)

Department of Pediatrics and Children's Healthcare of Atlanta, Emory University, Atlanta, Georgia, USA.
Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.

Evan J Anderson (EJ)

Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, Georgia, USA.
Department of Pediatrics and Children's Healthcare of Atlanta, Emory University, Atlanta, Georgia, USA.

Jesse J Waggoner (JJ)

Division of Infectious Diseases, Department of Medicine, Emory University, Atlanta, Georgia, USA.
Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.
Department of Global Health, Rollins School of Public Health, Atlanta, Georgia, USA.

Classifications MeSH