The complex, dynamic SpliceOme of the small GTPase transcripts altered by technique, sex, genetics, tissue specificity, and RNA base editing.
RNA modifications
cancer
expression
isoforms
protein modeling
small GTPase
splicing aberrations
Journal
Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250
Informations de publication
Date de publication:
2022
2022
Historique:
received:
31
08
2022
accepted:
01
11
2022
entrez:
5
12
2022
pubmed:
6
12
2022
medline:
6
12
2022
Statut:
epublish
Résumé
The small GTPase family is well-studied in cancer and cellular physiology. With 162 annotated human genes, the family has a broad expression throughout cells of the body. Members of the family have multiple exons that require splicing. Yet, the role of splicing within the family has been underexplored. We have studied the splicing dynamics of small GTPases throughout 41,671 samples by integrating Nanopore and Illumina sequencing techniques. Within this work, we have made several discoveries. 1). Using the GTEx long read data of 92 samples, each small GTPase gene averages two transcripts, with 83 genes (51%) expressing two or more isoforms. 2). Cross-tissue analysis of GTEx from 17,382 samples shows 41 genes (25%) expressing two or more protein-coding isoforms. These include protein-changing transcripts in genes such as
Identifiants
pubmed: 36467401
doi: 10.3389/fcell.2022.1033695
pii: 1033695
pmc: PMC9714508
doi:
Banques de données
figshare
['10.6084/m9.figshare.20371842']
Types de publication
Journal Article
Langues
eng
Pagination
1033695Subventions
Organisme : NCI NIH HHS
ID : K00 CA245821
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL153165
Pays : United States
Informations de copyright
Copyright © 2022 Das, Sherry, Vaughan, Henderson, Zieba, Uhl, Koehn, Bupp, Rajasekaran, Li, Chhetri, Nissim, Williams and Prokop.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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