Temporal changes in the microglial proteome of male and female mice after a diffuse brain injury using label-free quantitative proteomics.
TBI
biological sex
inflammation
microglia
proteomics
traumatic brain injury
Journal
Glia
ISSN: 1098-1136
Titre abrégé: Glia
Pays: United States
ID NLM: 8806785
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
revised:
22
11
2022
received:
27
05
2022
accepted:
22
11
2022
pubmed:
6
12
2022
medline:
17
2
2023
entrez:
5
12
2022
Statut:
ppublish
Résumé
Traumatic brain injury (TBI) triggers neuroinflammatory cascades mediated by microglia, which promotes tissue repair in the short-term. These cascades may exacerbate TBI-induced tissue damage and symptoms in the months to years post-injury. However, the progression of the microglial function across time post-injury and whether this differs between biological sexes is not well understood. In this study, we examined the microglial proteome at 3-, 7-, or 28-days after a midline fluid percussion injury (mFPI) in male and female mice using label-free quantitative proteomics. Data are available via ProteomeXchange with identifier PXD033628. We identified a reduction in microglial proteins involved with clearance of neuronal debris via phagocytosis at 3- and 7-days post-injury. At 28 days post-injury, pro-inflammatory proteins were decreased and anti-inflammatory proteins were increased in microglia. These results indicate a reduction in microglial clearance of neuronal debris in the days post-injury with a shift to anti-inflammatory function by 28 days following TBI. The changes in the microglial proteome that occurred across time post-injury did not differ between biological sexes. However, we did identify an increase in microglial proteins related to pro-inflammation and phagocytosis as well as insulin and estrogen signaling in males compared with female mice that occurred with or without a brain injury. Although the microglial response was similar between males and females up to 28 days following TBI, biological sex differences in the microglial proteome, regardless of TBI, has implications for the efficacy of treatment strategies targeting the microglial response post-injury.
Substances chimiques
Proteome
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
880-903Informations de copyright
© 2022 The Authors. GLIA published by Wiley Periodicals LLC.
Références
Neuropathol Appl Neurobiol. 2013 Oct;39(6):654-66
pubmed: 23231074
Glia. 2021 May;69(5):1292-1306
pubmed: 33492723
Mol Brain. 2021 Jan 27;14(1):25
pubmed: 33504361
J Neurotrauma. 2019 Apr 15;36(8):1318-1334
pubmed: 30398389
Brain Inj. 2008 Sep;22(10):740-51
pubmed: 18787983
Mol Neurobiol. 2021 Mar;58(3):1052-1061
pubmed: 33085047
Brain Behav Immun. 2012 Mar;26(3):419-28
pubmed: 22198120
Neuroscience. 2012 Dec 6;225:65-75
pubmed: 22960311
PLoS One. 2017 Sep 14;12(9):e0184811
pubmed: 28910378
J Neuropathol Exp Neurol. 2011 Jul;70(7):551-67
pubmed: 21666502
J Neurotrauma. 2019 Nov 15;36(22):3063-3091
pubmed: 30794028
J Neurosci. 2013 Feb 13;33(7):2761-72
pubmed: 23407936
Cell Rep. 2018 Sep 4;24(10):2773-2783.e6
pubmed: 30184509
Forensic Sci Int. 2004 Dec 16;146(2-3):97-104
pubmed: 15542269
Front Cell Neurosci. 2018 Dec 18;12:488
pubmed: 30618635
Neurorehabil Neural Repair. 2018 Feb;32(2):115-128
pubmed: 29554850
PLoS One. 2018 Aug 27;13(8):e0201878
pubmed: 30148836
Front Immunol. 2022 Feb 09;13:753570
pubmed: 35222368
J Neurosurg Pediatr. 2018 Jul;22(1):22-30
pubmed: 29676680
Brain Inj. 2016;30(11):1293-1301
pubmed: 27712117
Glia. 2023 Apr;71(4):880-903
pubmed: 36468604
Front Immunol. 2020 Jun 05;11:1110
pubmed: 32582192
Glia. 2002 Mar 15;37(4):314-27
pubmed: 11870871
Brain Struct Funct. 2012 Jan;217(1):49-61
pubmed: 21597967
Small GTPases. 2015;6(2):89-99
pubmed: 25941749
Glia. 2017 Sep;65(9):1423-1438
pubmed: 28608978
Brain Res Brain Res Rev. 1995 Mar;20(3):269-87
pubmed: 7550361
Mol Neurobiol. 2013 Dec;48(3):690-701
pubmed: 23613214
J Neurotrauma. 2021 Jun 1;38(11):1551-1571
pubmed: 33605175
Biol Psychiatry. 2014 Oct 1;76(7):575-84
pubmed: 24289885
J Neuroinflammation. 2021 Mar 17;18(1):72
pubmed: 33731173
Mol Cell Proteomics. 2020 Aug;19(8):1281-1302
pubmed: 32434922
Antioxidants (Basel). 2021 Oct 28;10(11):
pubmed: 34829586
J Clin Endocrinol Metab. 2009 Feb;94(2):518-22
pubmed: 19050054
Sci Signal. 2009 Jun 16;2(75):ra28
pubmed: 19531803
J Rehabil Med. 2004 Feb;(43 Suppl):84-105
pubmed: 15083873
J Neurol Neurosurg Psychiatry. 1999 Feb;66(2):207-13
pubmed: 10071101
Front Neurol. 2021 Sep 08;12:722526
pubmed: 34566867
J Neurotrauma. 2016 Oct 1;33(19):1732-1750
pubmed: 26486881
Mol Neurodegener. 2019 Jan 11;14(1):2
pubmed: 30634998
Int J Mol Sci. 2020 Dec 22;22(1):
pubmed: 33374952
Ann Neurol. 2011 Sep;70(3):374-83
pubmed: 21710619
Trends Neurosci. 2015 Oct;38(10):609-620
pubmed: 26442695
J Neurosci. 2011 Nov 9;31(45):16064-9
pubmed: 22072657
Int Immunopharmacol. 2021 Oct;99:107975
pubmed: 34293712
J Neurotrauma. 2021 Jan 15;38(2):235-251
pubmed: 32838645
Neurobiol Dis. 2021 May;152:105290
pubmed: 33556540
Sci Rep. 2015 Jun 08;5:10775
pubmed: 26053859
Front Mol Neurosci. 2022 Jan 25;14:785938
pubmed: 35145378
Endocr Connect. 2016 Jul;5(4):152-66
pubmed: 27317610
Methods Mol Biol. 2011;748:183-93
pubmed: 21701975
Front Cell Neurosci. 2019 Aug 08;13:307
pubmed: 31440141
Dev Neurosci. 2016;38(3):195-205
pubmed: 27449121
Sci Rep. 2017 Oct 16;7(1):13211
pubmed: 29038483
J Neuroinflammation. 2015 Oct 06;12:186
pubmed: 26438203
PLoS One. 2013 Apr 18;8(4):e61466
pubmed: 23637840
Nat Med. 2016 Sep;22(9):1002-12
pubmed: 27455510
J Neurotrauma. 2016 Apr 15;33(8):766-76
pubmed: 26437675
Toxicol Pathol. 2011 Jan;39(1):103-14
pubmed: 21078923
J Neurol. 2020 Nov;267(11):3223-3234
pubmed: 32535683
Brain Res. 2020 Aug 15;1741:146884
pubmed: 32422127
J Neuropathol Exp Neurol. 2007 Nov;66(11):989-1001
pubmed: 17984681
Nat Rev Immunol. 2018 Apr;18(4):225-242
pubmed: 29151590
J Clin Med. 2018 Mar 14;7(3):
pubmed: 29538298
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613
pubmed: 30476243
Shock. 2020 Jan;53(1):24-34
pubmed: 30939502
J Neuropathol Exp Neurol. 2014 Jan;73(1):14-29
pubmed: 24335533
PLoS One. 2019 Apr 3;14(4):e0214741
pubmed: 30943276
J Neurotrauma. 2019 Apr 1;36(7):1040-1053
pubmed: 30259790
J Neurotrauma. 2007 Feb;24(2):259-69
pubmed: 17375990
Brain Res. 2020 Nov 1;1746:146987
pubmed: 32592739
Front Immunol. 2020 Jul 16;11:1416
pubmed: 32765501
J Neurosci Rural Pract. 2012 May;3(2):131-5
pubmed: 22865961
Cell Rep. 2018 Jun 19;23(12):3501-3511
pubmed: 29924994
Neurobiol Dis. 2020 Mar;136:104713
pubmed: 31843705
Mol Neurodegener. 2018 Jun 28;13(1):34
pubmed: 29954413
J Neurotrauma. 2020 Oct 1;37(19):2120-2130
pubmed: 32326805
J Neuroinflammation. 2012 Oct 30;9:247
pubmed: 23111107
Front Cell Neurosci. 2013 Mar 18;7:26
pubmed: 23507975
J Neuroinflammation. 2021 Jan 18;18(1):24
pubmed: 33461596
Front Neurol. 2020 Sep 30;11:946
pubmed: 33101162
Front Neurol. 2019 Mar 08;10:198
pubmed: 30906278
Development. 2019 May 10;146(9):
pubmed: 31076485
Nucleic Acids Res. 2022 Jan 7;50(D1):D543-D552
pubmed: 34723319
Clin Immunol. 2021 Sep;230:108815
pubmed: 34339843
Front Neurosci. 2021 Oct 29;15:701317
pubmed: 34776838
Front Immunol. 2020 Mar 20;11:493
pubmed: 32265936
Brain Behav Immun. 2018 Feb;68:183-196
pubmed: 29061364
Front Neurol. 2022 Jan 17;12:804139
pubmed: 35111130
Methods Mol Biol. 2016;1462:211-30
pubmed: 27604721
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
Brain. 2013 Jan;136(Pt 1):28-42
pubmed: 23365092
Med Sci Monit. 2010 Dec;16(12):CR593-7
pubmed: 21119577
EMBO J. 2019 Sep 2;38(17):e101997
pubmed: 31373067
Metab Brain Dis. 2015 Apr;30(2):393-400
pubmed: 24733573
Front Cell Neurosci. 2019 Oct 10;13:457
pubmed: 31649511
Nat Protoc. 2019 Jan;14(1):68-85
pubmed: 30464214
Front Neurol. 2018 Nov 15;9:964
pubmed: 30498469
Mol Cell Biol. 2000 Jun;20(11):4106-14
pubmed: 10805752
JAMA Neurol. 2019 Sep 01;76(9):1049-1059
pubmed: 31157856
Curr Alzheimer Res. 2015;12(7):684-93
pubmed: 26159205
Sci Rep. 2018 May 1;8(1):6807
pubmed: 29717219
Neuron. 2017 Sep 13;95(6):1246-1265
pubmed: 28910616
Dis Model Mech. 2013 Nov;6(6):1307-15
pubmed: 24046353
Science. 2005 May 27;308(5726):1314-8
pubmed: 15831717
Biostatistics. 2009 Jul;10(3):515-34
pubmed: 19377034
PLoS One. 2016 Jan 25;11(1):e0148001
pubmed: 26808663
Mol Psychiatry. 2019 Mar;24(3):431-446
pubmed: 30283032
J Neurosci. 2021 Feb 17;41(7):1597-1616
pubmed: 33452227