Intranuclear inclusions in muscle biopsy can differentiate oculopharyngodistal myopathy and oculopharyngeal muscular dystrophy.

CGG repeat expansion GIPC1 Intranuclear inclusion LRP12 NOTCH2NLC Oculopharyngeal muscular dystrophy Oculopharyngodistal myopathy

Journal

Acta neuropathologica communications
ISSN: 2051-5960
Titre abrégé: Acta Neuropathol Commun
Pays: England
ID NLM: 101610673

Informations de publication

Date de publication:
07 12 2022
Historique:
received: 01 10 2022
accepted: 18 11 2022
entrez: 8 12 2022
pubmed: 9 12 2022
medline: 15 12 2022
Statut: epublish

Résumé

Oculopharyngodistal myopathy (OPDM) and oculopharyngeal muscular dystrophy (OPMD) are similar and even believed to be indistinguishable in terms of their myopathological features. To address the diagnostic gap, we evaluated the muscle biopsy samples for p62 expression by immunohistochemistry and compared the occurrence and the frequency of intranuclear inclusions among the individuals with OPDM (harboring CGG repeat expansion in LRP12 (n = 19), GIPC1 (n = 6), or NOTCH2NLC (n = 7)), OPMD (n = 15), and other rimmed vacuolar myopathies. We found that myonuclei with p62-positive intra-nuclear inclusions (myo-INIs) were significantly more frequent in OPMD (11.9 ± 1.1%, range 5.9-18.6%) than in OPDM and other rimmed vacuolar myopathies (RVMs) (0.9-1.5% on average, range 0.0-2.8%, p < 0.0001). In contrast, INIs in non-muscle cells such as blood vessels, peripheral nerve bundles, and muscle spindles (non-muscle-INIs) were present in OPDM, but absent in OPMD. These results indicate that OPMD can be differentiated from OPDM and other RVMs by the frequent presence of myo-INIs; and in OPDM, the presence of non-muscle-INIs in muscle pathology should be a diagnostic hallmark.

Identifiants

pubmed: 36476314
doi: 10.1186/s40478-022-01482-w
pii: 10.1186/s40478-022-01482-w
pmc: PMC9727945
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

176

Informations de copyright

© 2022. The Author(s).

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Auteurs

Masashi Ogasawara (M)

Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan.
Medical Genome Center, NCNP, Kodaira, Tokyo, Japan.
Department of Pediatrics, Showa General Hospital, Kodaira, Tokyo, Japan.

Nobuyuki Eura (N)

Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan.
Medical Genome Center, NCNP, Kodaira, Tokyo, Japan.
Department of Neurology, Nara Medical University, Nara, Japan.

Aritoshi Iida (A)

Medical Genome Center, NCNP, Kodaira, Tokyo, Japan.

Theerawat Kumutpongpanich (T)

Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan.
Medical Genome Center, NCNP, Kodaira, Tokyo, Japan.

Narihiro Minami (N)

Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan.
Medical Genome Center, NCNP, Kodaira, Tokyo, Japan.

Ikuya Nonaka (I)

Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan.

Shinichiro Hayashi (S)

Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan.
Medical Genome Center, NCNP, Kodaira, Tokyo, Japan.

Satoru Noguchi (S)

Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan.
Medical Genome Center, NCNP, Kodaira, Tokyo, Japan.

Ichizo Nishino (I)

Department of Neuromuscular Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawahigashi, Kodaira, Tokyo, 187-8502, Japan. nishino@ncnp.go.jp.
Medical Genome Center, NCNP, Kodaira, Tokyo, Japan. nishino@ncnp.go.jp.

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