Characterization of an atypical Actinobacillus pleuropneumoniae serovar 2 isolate with a rough-type lipopolysaccharide.
Actinobacillus pleuropneumoniae
atypical
rough-type lipopolysaccharide
swine
wcaJ
Journal
The Journal of veterinary medical science
ISSN: 1347-7439
Titre abrégé: J Vet Med Sci
Pays: Japan
ID NLM: 9105360
Informations de publication
Date de publication:
01 Feb 2023
01 Feb 2023
Historique:
pubmed:
9
12
2022
medline:
4
2
2023
entrez:
8
12
2022
Statut:
ppublish
Résumé
We describe phenotypic and genetic characterization of an atypical Japanese Actinobacillus pleuropneumoniae isolate OT761. Nucleotide sequence analysis revealed that gene clusters involved in capsular polysaccharide and O-polysaccharide (O-PS) biosynthesis of the isolate were nearly identical to those of serovar 2 reference strain. The main difference found between the O-PS loci is the shortening of 31 amino acids from the C terminus of WcaJ in the atypical isolate due to a 93 bp deletion at the 3' end of wcaJ gene. Immunoblot analysis revealed that this isolate could not produce O-PS. Taken together, our results showed that the C-terminal domain of the A. pleuropneumoniae WcaJ plays a critical role in enzyme function of WcaJ involved in the biosynthesis of O-PS.
Identifiants
pubmed: 36477365
doi: 10.1292/jvms.22-0258
pmc: PMC10017293
doi:
Substances chimiques
Lipopolysaccharides
0
Polysaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
157-162Références
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