Translational opportunities of single-cell biology in atherosclerosis.
Atherosclerosis
Endothelial cell
Lymphocyte
Macrophage
Single-cell biology
Smooth muscle cell
Journal
European heart journal
ISSN: 1522-9645
Titre abrégé: Eur Heart J
Pays: England
ID NLM: 8006263
Informations de publication
Date de publication:
07 04 2023
07 04 2023
Historique:
received:
31
03
2022
revised:
28
10
2022
accepted:
10
11
2022
medline:
10
4
2023
pubmed:
9
12
2022
entrez:
8
12
2022
Statut:
ppublish
Résumé
The advent of single-cell biology opens a new chapter for understanding human biological processes and for diagnosing, monitoring, and treating disease. This revolution now reaches the field of cardiovascular disease (CVD). New technologies to interrogate CVD samples at single-cell resolution are allowing the identification of novel cell communities that are important in shaping disease development and direct towards new therapeutic strategies. These approaches have begun to revolutionize atherosclerosis pathology and redraw our understanding of disease development. This review discusses the state-of-the-art of single-cell analysis of atherosclerotic plaques, with a particular focus on human lesions, and presents the current resolution of cellular subpopulations and their heterogeneity and plasticity in relation to clinically relevant features. Opportunities and pitfalls of current technologies as well as the clinical impact of single-cell technologies in CVD patient care are highlighted, advocating for multidisciplinary and international collaborative efforts to join the cellular dots of CVD.
Identifiants
pubmed: 36478058
pii: 6881119
doi: 10.1093/eurheartj/ehac686
pmc: PMC10120164
doi:
Types de publication
Review
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1216-1230Subventions
Organisme : Medical Research Council
ID : MR/W00366X/1
Pays : United Kingdom
Organisme : NHLBI NIH HHS
ID : R01 HL146465
Pays : United States
Organisme : British Heart Foundation
ID : RG/19/10/34506
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of the European Society of Cardiology.
Déclaration de conflit d'intérêts
Conflict of interest M.P.J.W. is funded by The Netherlands Heart Foundation (CVON 2017–20); The Netherlands Heart Foundation and Spark-Holding BV [2019B016]; Foundation Leducq (LEAN Transatlantic Network Grant); Amsterdam Cardiovascular Sciences; Amsterdam UMC; ZonMW (Open competition 09120011910025). P.E. is supported by the British Heart Foundation [RG/19/10/34506]. D.G. is funded by the National Institute of Health R01HL146465 and the American Heart Association 20IPA35310394 grants. I.G. is supported by the Swedish Research Council, the Swedish Heart and Lung Foundation, Skåne University Hospital funds, Lund University Diabetes Center (the Swedish Foundation for Strategic Research Dnr IRC15-006) and Region Skåne. H.F.J. is funded by the British Heart Foundation [PG/19/6/34153, RM/13/3/30159]. E.L. is supported by the European Research Council (ERC consolidator grant) and the Deutsche Forschungsgemeinschaft [CRC 1123]. G.D.N. is supported by: Telethon Foundation [GGP19146], Progetti di Rilevante Interesse Nazionale [PRIN 2017 K55HLC], Ricerca Finalizzata, Ministry of Health [RF-2019-12370896], and PNRR Missione 4 [Progetto CN3—National Center for Gene Therapy and Drugs based on RNA Technology]. E.O. is supported by the Swiss National Science Foundation (PRIMA: PR00P3_179861/1), the Swiss Life Foundation, the Alfred and Annemarie von Sick Grants for Translational and Clinical Research Cardiology and Oncology, the Heubergstiftung and the Swiss Heart Foundation. M.S. is funded by NIH grant HL139582. S.Y.H. is funded by the European Research Council Advanced Grant No 844382. H.W. is supported by the Neven-DuMont Foundation and the Deutsche Forschungsgemeinschaft: SFB TRR259 [397484323] and GRK2407 [360043781]. M.L.B.P. is supported by the Swiss National Science Foundation # 310030_185370/1. C.M. is funded by the British Heart Foundation [PG/18/1/33430 and PG/19/41/34426], the European Commission (TAXINOMISIS; grant agreement H2020-SC1-2016-2017, and 797788 STRIKING STREAKS), The Kennedy Trustees, Novo Nordisk (Oxford Novo Nordisk Fellowship), and the Novo Nordisk Foundation [NNF15CC0018346 and NNF0064142]. Figures were made with BioRender.
Références
Nat Med. 2019 Oct;25(10):1576-1588
pubmed: 31591603
Nat Rev Cardiol. 2019 Dec;16(12):727-744
pubmed: 31243391
Immunity. 2015 Jun 16;42(6):1100-15
pubmed: 26084025
J Vis Exp. 2011 Jul 01;(53):
pubmed: 21750492
Nat Immunol. 2016 Feb;17(2):159-68
pubmed: 26642357
Nat Commun. 2022 Jan 11;13(1):215
pubmed: 35017526
Immunity. 2018 Aug 21;49(2):326-341.e7
pubmed: 30054204
Nat Immunol. 2019 Feb;20(2):163-172
pubmed: 30643263
Cardiovasc Res. 2018 Mar 15;114(4):540-550
pubmed: 29385543
Cells. 2020 Dec 11;9(12):
pubmed: 33322482
Mol Cell. 1998 Aug;2(2):275-81
pubmed: 9734366
Circulation. 2020 Nov 24;142(21):2045-2059
pubmed: 32674599
Circulation. 2021 Feb 16;143(7):713-726
pubmed: 33499648
Nat Med. 2019 Aug;25(8):1280-1289
pubmed: 31359001
Nat Commun. 2018 Nov 1;9(1):4567
pubmed: 30385745
Nat Biotechnol. 2021 Sep;39(9):1103-1114
pubmed: 33349700
Cells. 2021 Feb 19;10(2):
pubmed: 33669769
Circ Res. 2018 Jun 8;122(12):1661-1674
pubmed: 29545365
J Am Heart Assoc. 2015 Mar 26;4(4):
pubmed: 25814626
J Clin Invest. 2015 Oct 26;125(12):4514-28
pubmed: 26517696
J Am Coll Cardiol. 2019 Jan 22;73(2):190-209
pubmed: 30654892
Mol Cell. 2017 Feb 16;65(4):631-643.e4
pubmed: 28212749
Circulation. 2020 Nov 24;142(21):2060-2075
pubmed: 32962412
Circulation. 2020 Sep 29;142(13):1279-1293
pubmed: 32703007
Circulation. 2020 Oct 6;142(14):1374-1388
pubmed: 33017217
Atherosclerosis. 2022 Jan;340:12-22
pubmed: 34871816
Cell Rep. 2020 Dec 15;33(11):108491
pubmed: 33326796
Nat Methods. 2021 Apr;18(4):327-328
pubmed: 33782609
Nat Rev Genet. 2015 Mar;16(3):133-45
pubmed: 25628217
Nat Commun. 2021 Jan 29;12(1):681
pubmed: 33514719
Nat Methods. 2015 May;12(5):453-7
pubmed: 25822800
Arteriosclerosis. 1986 Mar-Apr;6(2):131-8
pubmed: 2937395
Arterioscler Thromb Vasc Biol. 2019 Jan;39(1):25-36
pubmed: 30580568
Arterioscler Thromb Vasc Biol. 2022 Apr;42(4):e86-e95
pubmed: 35139657
Genome Biol. 2020 Jun 2;21(1):130
pubmed: 32487174
Eur Heart J. 2022 May 14;43(19):1864-1877
pubmed: 35567557
Nature. 2019 Nov;575(7783):512-518
pubmed: 31597160
Cardiovasc Res. 2019 Oct 1;115(12):1705-1715
pubmed: 31350876
Cells. 2020 Nov 30;9(12):
pubmed: 33266027
Nat Commun. 2017 Feb 09;8:14361
pubmed: 28181491
Nat Rev Cardiol. 2020 Jul;17(7):387-401
pubmed: 32203286
Nat Commun. 2016 Jun 24;7:11853
pubmed: 27340017
Nat Protoc. 2021 Jan;16(1):1-9
pubmed: 33288955
Cell. 2020 Sep 3;182(5):1232-1251.e22
pubmed: 32822576
Arterioscler Thromb Vasc Biol. 2020 Feb;40(2):309-322
pubmed: 31852222
Circulation. 2014 Apr 15;129(15):1551-9
pubmed: 24481950
Immunometabolism. 2021;3(3):
pubmed: 34178388
Arterioscler Thromb Vasc Biol. 2000 May;20(5):1262-75
pubmed: 10807742
Sci Signal. 2014 Sep 23;7(344):ra90
pubmed: 25249657
Nat Biotechnol. 2020 Jun;38(6):747-755
pubmed: 32518403
Circulation. 2018 Sep 11;138(11):1130-1143
pubmed: 29588316
Circulation. 2019 Jul 9;140(2):147-163
pubmed: 31146585
Circ Res. 2020 Apr 24;126(9):1112-1126
pubmed: 32324494
Science. 2018 Apr 20;360(6386):270-271
pubmed: 29674582
Arterioscler Thromb Vasc Biol. 2021 Sep;41(9):2357-2369
pubmed: 34196216
Eur Heart J. 2021 Jan 1;42(1):113-131
pubmed: 32176778
Nat Rev Cardiol. 2020 Jan;17(1):52-63
pubmed: 31366922
Cardiovasc Res. 2021 Apr 23;117(5):1402-1416
pubmed: 32678909
Circ Res. 2020 Jul 17;127(3):402-426
pubmed: 32673538
J Exp Med. 2006 May 15;203(5):1273-82
pubmed: 16682495
Nature. 2021 Apr;592(7855):524-533
pubmed: 33883728
Proc Natl Acad Sci U S A. 2019 Apr 2;116(14):6784-6789
pubmed: 30872485
Sci Rep. 2018 Feb 13;8(1):2919
pubmed: 29440771
Nat Rev Cardiol. 2015 Jan;12(1):10-7
pubmed: 25367649
Nat Commun. 2019 Aug 29;10(1):3908
pubmed: 31467286
N Engl J Med. 2017 Sep 21;377(12):1119-1131
pubmed: 28845751
Eur Heart J Open. 2021 Dec 21;2(1):oeab043
pubmed: 35174364
Nat Rev Cardiol. 2022 Aug;19(8):522-542
pubmed: 35102320
Circ Res. 2016 Dec 9;119(12):1313-1323
pubmed: 27682618
Atherosclerosis. 2012 Dec;225(2):461-8
pubmed: 23078881
Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):15818-15826
pubmed: 32541024
Cell Stem Cell. 2016 Nov 3;19(5):628-642
pubmed: 27618218
Nat Methods. 2017 Sep 29;14(10):935-936
pubmed: 28960196
Nat Biotechnol. 2020 Jun;38(6):737-746
pubmed: 32341560
Circ Res. 2020 Nov 6;127(11):1437-1455
pubmed: 32981416
Nature. 2020 Oct;586(7831):785-789
pubmed: 33057196
Cells. 2020 Dec 29;10(1):
pubmed: 33383733
Sci Adv. 2021 Jul 9;7(28):
pubmed: 34244146
Nat Methods. 2019 Jun;16(6):479-487
pubmed: 31133762
Nat Metab. 2021 Feb;3(2):166-181
pubmed: 33619382
Circ Res. 2018 Jun 8;122(12):1675-1688
pubmed: 29545366
Circ Res. 2014 Sep 12;115(7):662-7
pubmed: 25070003
J Am Soc Nephrol. 2019 Jan;30(1):23-32
pubmed: 30510133
RNA. 2017 Jun;23(6):860-865
pubmed: 28258159
Front Immunol. 2018 Oct 23;9:2425
pubmed: 30405621
Genome Biol. 2016 Apr 29;17:80
pubmed: 27139883
Cell. 2019 Jul 25;178(3):686-698.e14
pubmed: 31257031
Nat Neurosci. 2019 Jun;22(6):1021-1035
pubmed: 31061494
Nat Methods. 2021 Jan;18(1):9-14
pubmed: 33408395
Circulation. 1995 Sep 1;92(5):1355-74
pubmed: 7648691
Nat Rev Cardiol. 2019 Mar;16(3):180-196
pubmed: 30410107
Nat Biotechnol. 2020 Mar;38(3):254-257
pubmed: 32152578
J Clin Invest. 2021 Feb 15;131(4):
pubmed: 33586673
Am J Pathol. 1997 May;150(5):1687-99
pubmed: 9137093
Nat Rev Cardiol. 2022 Jan;19(1):43-58
pubmed: 34267377
Biochem Soc Trans. 2021 Nov 1;49(5):2101-2111
pubmed: 34495326
Sci Rep. 2017 Jun 13;7(1):3375
pubmed: 28611395
J Pathol. 2020 Apr;250(5):705-714
pubmed: 32003464
Arterioscler Thromb Vasc Biol. 2021 Apr;41(4):1408-1427
pubmed: 33626908
Cell Stem Cell. 2020 Apr 2;26(4):542-557.e11
pubmed: 32243809
Lancet. 2020 Oct 17;396(10258):1204-1222
pubmed: 33069326
JCI Insight. 2019 Feb 21;4(4):
pubmed: 30830865
Circ Res. 2021 Jul 9;129(2):240-258
pubmed: 34024118
Nat Metab. 2019 Sep;1(9):912-926
pubmed: 31572976
Circ Res. 2018 Oct 26;123(10):1127-1142
pubmed: 30359200
Nat Med. 2015 Jun;21(6):628-37
pubmed: 25985364
Nat Immunol. 2020 Sep;21(9):1107-1118
pubmed: 32788748