The piRNA pathway is required for nucleopolyhedrovirus replication in Lepidoptera.
BmAgo3
Bombyx mori
Piwi-interacting RNA
Siwi
nucleopolyhedrovirus
Journal
Insect science
ISSN: 1744-7917
Titre abrégé: Insect Sci
Pays: Australia
ID NLM: 101266965
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
revised:
07
11
2022
received:
28
07
2022
accepted:
22
11
2022
pubmed:
11
12
2022
medline:
11
12
2022
entrez:
10
12
2022
Statut:
ppublish
Résumé
The Piwi-interacting RNA (piRNA) pathway has been shown to be involved in the antiviral defense against RNA viruses, especially in mosquitoes, but its universality has been questioned. Here, we used the Bombyx mori nucleopolyhedrovirus (BmNPV) -infected silkworm as a model to explore the effects of the key factors of piRNA pathway, BmAgo3 and Siwi, on replication of a large DNA virus (belonging to the family of Baculoviridae). We demonstrated that BmAgo3 and Siwi could promote the replication of BmNPV through both overexpression and knockdown experiments in BmN cell lines and silkworm larvae. In addition, we also studied the effect of PIWI-class genes on Autographa californica nucleopolyhedrovirus (AcMNPV) replication in the Spodoptera frugiperda cell line Sf9. By knocking down the expression of PIWI-class genes in Sf9, we found that Piwi-like-1 and Piwi-like-2-3 could inhibit AcMNPV replication, while Piwi-like-4-5 promoted virus replication. Our study provides compelling evidence that the piRNA pathway affects host infection by exogenous viruses in Lepidoptera. Also, our results reflect the diversity of the roles of PIWI-class genes in virus infection of the host across species. This study is the first to explore the interaction of PIWI-class proteins with DNA viruses, providing new insights into the functional roles of the piRNA pathway.
Identifiants
pubmed: 36495071
doi: 10.1111/1744-7917.13160
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1378-1392Subventions
Organisme : Guangzhou Science and Technology Plan
ID : 202201010369
Organisme : Natural Science Foundation of Guangdong Basic and Applied Basic Research Fund
ID : 2022A1515012657
Organisme : National Natural Science Foundation of China
ID : 31872426
Organisme : South China Agricultural University high-level talent launch project
Organisme : Guangdong Provincial Promotion Project on Preservation
Organisme : Utilization of Local Breed of Livestock and Poultry
ID : 2018-143
Informations de copyright
© 2022 Institute of Zoology, Chinese Academy of Sciences.
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