Incidence and severity of immune-related hepatitis after dual checkpoint therapy is linked to younger age independent of herpes virus immunity.

Age Checkpoint therapy Hepatitis Herpes virus Immune-related adverse effects

Journal

Journal of translational medicine
ISSN: 1479-5876
Titre abrégé: J Transl Med
Pays: England
ID NLM: 101190741

Informations de publication

Date de publication:
12 Dec 2022
Historique:
received: 06 10 2022
accepted: 04 11 2022
entrez: 12 12 2022
pubmed: 13 12 2022
medline: 15 12 2022
Statut: epublish

Résumé

Dual immune checkpoint blockade (ICB) therapy can result in immune-related-adverse events (irAE) such as ICB-hepatitis. An expansion of effector-memory (TEM) CD4 T cells associated with antiviral immunity against herpesviridae was implicated in ICB-hepatitis. Notably, these memory subsets are frequently associated with age. Here, we sought to understand baseline patient, immune and viral biomarkers associated with the development of ICB-hepatitis to identify currently lacking baseline predictors and test if an expansion of TEM or positive serology against herpesviridae can predict ICB-hepatitis. A discovery (n = 39) and validation cohort (n = 67) of patients with advanced melanoma undergoing anti-PD-1&anti-CTLA4 combination therapy (total n = 106) were analyzed for baseline clinical characteristics, occurrence of irAE and oncological outcomes alongside serological status for CMV, EBV and HSV. Immune populations were profiled by high-parametric flow cytometry (n = 29). ICB-hepatitis occurred in 59% of patients within 100 days; 35.9% developed severe (CTCAE 3-4) hepatitis. Incidence of ICB-hepatitis was higher in the younger (< 55y: 85.7%) compared to older (> = 55y: 27.8%) age group (p = 0.0003), occured earlier in younger patients (p < 0.0001). The association of younger age with ICB-Hepatitis was also observed in the validation cohort (p = 0.0486). Incidence of ICB-hepatitis was also associated with additional non-hepatic irAE (p = 0.018), but neither positive IgG serostatus for CMV, EBV or HSV nor TEM subsets despite an association of T cell subsets with age. Younger age more accurately predicts ICB-hepatitis after anti-PD-1&anti-CTLA4 checkpoint therapy at baseline compared to herpes virus serology or TEM subsets. Younger patients should be carefully monitored for the development of ICB-hepatitis.

Sections du résumé

BACKGROUND AND AIMS OBJECTIVE
Dual immune checkpoint blockade (ICB) therapy can result in immune-related-adverse events (irAE) such as ICB-hepatitis. An expansion of effector-memory (TEM) CD4 T cells associated with antiviral immunity against herpesviridae was implicated in ICB-hepatitis. Notably, these memory subsets are frequently associated with age. Here, we sought to understand baseline patient, immune and viral biomarkers associated with the development of ICB-hepatitis to identify currently lacking baseline predictors and test if an expansion of TEM or positive serology against herpesviridae can predict ICB-hepatitis.
METHODS METHODS
A discovery (n = 39) and validation cohort (n = 67) of patients with advanced melanoma undergoing anti-PD-1&anti-CTLA4 combination therapy (total n = 106) were analyzed for baseline clinical characteristics, occurrence of irAE and oncological outcomes alongside serological status for CMV, EBV and HSV. Immune populations were profiled by high-parametric flow cytometry (n = 29).
RESULTS RESULTS
ICB-hepatitis occurred in 59% of patients within 100 days; 35.9% developed severe (CTCAE 3-4) hepatitis. Incidence of ICB-hepatitis was higher in the younger (< 55y: 85.7%) compared to older (> = 55y: 27.8%) age group (p = 0.0003), occured earlier in younger patients (p < 0.0001). The association of younger age with ICB-Hepatitis was also observed in the validation cohort (p = 0.0486). Incidence of ICB-hepatitis was also associated with additional non-hepatic irAE (p = 0.018), but neither positive IgG serostatus for CMV, EBV or HSV nor TEM subsets despite an association of T cell subsets with age.
CONCLUSION CONCLUSIONS
Younger age more accurately predicts ICB-hepatitis after anti-PD-1&anti-CTLA4 checkpoint therapy at baseline compared to herpes virus serology or TEM subsets. Younger patients should be carefully monitored for the development of ICB-hepatitis.

Identifiants

pubmed: 36503532
doi: 10.1186/s12967-022-03755-3
pii: 10.1186/s12967-022-03755-3
pmc: PMC9743538
doi:

Substances chimiques

Biomarkers 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

582

Subventions

Organisme : DFG
ID : 256073931
Organisme : DFG
ID : 441891347
Organisme : DFG
ID : 413517907
Organisme : DFG
ID : 378189018

Informations de copyright

© 2022. The Author(s).

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Auteurs

Zhen Zhang (Z)

Faculty of Medicine, Clinic for Internal Medicine II, Gastroenterology, Hepatology, Endocrinology, and Infectious Disease, University Medical Center Freiburg, Freiburg, Germany.

David Rafei-Shamsabadi (D)

Faculty of Medicine, Department of Dermatology and Venereology, University Medical Center Freiburg, Freiburg, Germany.

Saskia Lehr (S)

Faculty of Medicine, Department of Dermatology and Venereology, University Medical Center Freiburg, Freiburg, Germany.

Nico Buettner (N)

Faculty of Medicine, Clinic for Internal Medicine II, Gastroenterology, Hepatology, Endocrinology, and Infectious Disease, University Medical Center Freiburg, Freiburg, Germany.

Rebecca Diehl (R)

Faculty of Medicine, Department of Dermatology and Venereology, University Medical Center Freiburg, Freiburg, Germany.

Daniela Huzly (D)

Institute of Virology, Faculty of Medicine, Freiburg University Medical Center, University of Freiburg, Freiburg, Germany.

David J Pinato (DJ)

Department of Surgery and Cancer, Imperial College London, Hammersmith Hospital, London, UK.
Department of Translational Medicine, University of Piemonte Orientale, Novara, Italy.

Robert Thimme (R)

Faculty of Medicine, Clinic for Internal Medicine II, Gastroenterology, Hepatology, Endocrinology, and Infectious Disease, University Medical Center Freiburg, Freiburg, Germany.

Frank Meiss (F)

Faculty of Medicine, Department of Dermatology and Venereology, University Medical Center Freiburg, Freiburg, Germany.

Bertram Bengsch (B)

Faculty of Medicine, Clinic for Internal Medicine II, Gastroenterology, Hepatology, Endocrinology, and Infectious Disease, University Medical Center Freiburg, Freiburg, Germany. Bertram.bengsch@uniklinik-freiburg.de.
Signalling Research Centres BIOSS and CIBSS, University of Freiburg, Freiburg, Germany. Bertram.bengsch@uniklinik-freiburg.de.
Partner Site Freiburg, German Cancer Consortium (DKTK), Heidelberg, Germany. Bertram.bengsch@uniklinik-freiburg.de.

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