Engineered Spider Silk Proteins for Biomimetic Spinning of Fibers with Toughness Equal to Dragline Silks.
biomimetic materials
biomimetic spider silk fibers
fibers
protein engineering
recombinant protein production
Journal
Advanced functional materials
ISSN: 1616-301X
Titre abrégé: Adv Funct Mater
Pays: Germany
ID NLM: 101190390
Informations de publication
Date de publication:
03 Jun 2022
03 Jun 2022
Historique:
received:
24
01
2022
revised:
10
03
2022
entrez:
12
12
2022
pubmed:
13
12
2022
medline:
13
12
2022
Statut:
ppublish
Résumé
Spider silk is the toughest fiber found in nature, and bulk production of artificial spider silk that matches its mechanical properties remains elusive. Development of miniature spider silk proteins (mini-spidroins) has made large-scale fiber production economically feasible, but the fibers' mechanical properties are inferior to native silk. The spider silk fiber's tensile strength is conferred by poly-alanine stretches that are zipped together by tight side chain packing in β-sheet crystals. Spidroins are secreted so they must be void of long stretches of hydrophobic residues, since such segments get inserted into the endoplasmic reticulum membrane. At the same time, hydrophobic residues have high β-strand propensity and can mediate tight inter-β-sheet interactions, features that are attractive for generation of strong artificial silks. Protein production in prokaryotes can circumvent biological laws that spiders, being eukaryotic organisms, must obey, and the authors thus design mini-spidroins that are predicted to more avidly form stronger β-sheets than the wildtype protein. Biomimetic spinning of the engineered mini-spidroins indeed results in fibers with increased tensile strength and two fiber types display toughness equal to native dragline silks. Bioreactor expression and purification result in a protein yield of ≈9 g L
Identifiants
pubmed: 36505976
doi: 10.1002/adfm.202200986
pii: ADFM202200986
pmc: PMC9720699
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2200986Informations de copyright
© 2022 The Authors. Advanced Functional Materials published by Wiley‐VCH GmbH.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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