Homopurine guanine-rich sequences in complex with N-methyl mesoporphyrin IX form parallel G-quadruplex dimers and display a unique symmetry tetrad.
Binding constant
G-quadruplex
Homopurine sequences
N-methyl mesoporphyrin IX
Symmetry tetrad
X-ray crystallography
Journal
Bioorganic & medicinal chemistry
ISSN: 1464-3391
Titre abrégé: Bioorg Med Chem
Pays: England
ID NLM: 9413298
Informations de publication
Date de publication:
01 01 2023
01 01 2023
Historique:
received:
20
09
2022
revised:
24
11
2022
accepted:
28
11
2022
pmc-release:
01
01
2024
pubmed:
13
12
2022
medline:
4
1
2023
entrez:
12
12
2022
Statut:
ppublish
Résumé
DNA can fold into G-quadruplexes (GQs), non-canonical secondary structures formed by π-π stacking of G-tetrads. GQs are important in many biological processes, which makes them promising therapeutic targets. We identified a 42-nucleotide long, purine-only G-rich sequence from human genome, which contains eight G-stretches connected by A and AAAA loops. We divided this sequence into five unique segments, four guanine stretches each, named GA1-5. In order to investigate the role of adenines in GQ structure formation, we performed biophysical and X-ray crystallographic studies of GA1-5 and their complexes with a highly selective GQ ligand, N-methyl mesoporphyrin IX (NMM). Our data indicate that all variants form parallel GQs whose stability depends on the number of flexible AAAA loops. GA1-3 bind NMM with 1:1 stoichiometry. The K
Identifiants
pubmed: 36508994
pii: S0968-0896(22)00505-3
doi: 10.1016/j.bmc.2022.117112
pmc: PMC9812923
mid: NIHMS1858788
pii:
doi:
Substances chimiques
mesoporphyrin IX
493-90-3
Guanine
5Z93L87A1R
Mesoporphyrins
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
117112Subventions
Organisme : NCI NIH HHS
ID : R15 CA253134
Pays : United States
Organisme : NIGMS NIH HHS
ID : P30 GM124165
Pays : United States
Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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