The regional distribution of resident immune cells shapes distinct immunological environments along the murine epididymis.
bacterial infection
epididymis
epididymitis
immunology
inflammation
macrophages
mouse
resident immune cells
scRNASeq
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
14 12 2022
14 12 2022
Historique:
received:
26
07
2022
accepted:
29
11
2022
entrez:
14
12
2022
pubmed:
15
12
2022
medline:
17
12
2022
Statut:
epublish
Résumé
The epididymis functions as transition zone for post-testicular sperm maturation and storage and faces contrasting immunological challenges, i.e. tolerance towards spermatozoa vs. reactivity against pathogens. Thus, normal organ function and integrity relies heavily on a tightly controlled immune balance. Previous studies described inflammation-associated tissue damage solely in the distal regions (corpus, cauda), but not in the proximal regions (initial segment, caput). To understand the observed region-specific immunity along the epididymal duct, we have used an acute bacterial epididymitis mouse model and analyzed the disease progression. Whole transcriptome analysis using RNAseq 10 days post infection showed a pro-inflammatory environment within the cauda, while the caput exhibited only minor transcriptional changes. High-dimensional flow cytometry analyses revealed drastic changes in the immune cell composition upon infection with uropathogenic
Identifiants
pubmed: 36515584
doi: 10.7554/eLife.82193
pii: 82193
pmc: PMC9750176
doi:
pii:
Banques de données
GEO
['GSE208244']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2022, Pleuger et al.
Déclaration de conflit d'intérêts
CP, DA, MH, LW, DB, DK, SG, SE, CK, MF, SR, RM, JM, KL, MH, SB, AM No competing interests declared
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