Human Pre-gastrulation Embryo Culture in 3D Condition.

Human embryo Pre-gastrulation Primitive streak Three-dimensional (3D) culture

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
15 Dec 2022
Historique:
entrez: 14 12 2022
pubmed: 15 12 2022
medline: 15 12 2022
Statut: aheadofprint

Résumé

The development process of human embryo until blastocyst is well understood during the past 30 years, however, embryogenesis from blastocyst to pre-gastrulation was still remained a "black box". Limited by research materials and culture technologies, the "black box" is still unopened. We recently established an extended three-dimensional (3D) culture system of human blastocysts (Xiang et al., Nature 577(7791):537-542, 2020). The 3D embryo culture system could enable human blastocyst growing up to early primitive streak anlage stage in vitro. Here, we introduce the detail protocol and notes of culturing human 3D embryos.

Identifiants

DOI: 10.1007/7651_2022_466 PMID: 36515895
pubmed: 36515895
doi: 10.1007/7651_2022_466
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© 2023. Springer Science+Business Media, LLC.

Références

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Auteurs

Lifeng Xiang (L)

Department of Reproductive Medicine, The First People's Hospital of Yunnan Province, Kunming, China.
State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.

Yu Yin (Y)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.

Gaohui Shi (G)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China.

Tianqing Li (T)

State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan, China. litq@lpbr.cn.

Classifications MeSH