The injured sciatic nerve atlas (iSNAT), insights into the cellular and molecular basis of neural tissue degeneration and regeneration.
Wallerian degeneration
immunology
inflammation
mouse
neuroscience
parabiosis
regeneration
sciatic nerve
single-cell RNA seq
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
14 12 2022
14 12 2022
Historique:
received:
08
06
2022
accepted:
30
11
2022
pubmed:
15
12
2022
medline:
12
1
2023
entrez:
14
12
2022
Statut:
epublish
Résumé
Upon trauma, the adult murine peripheral nervous system (PNS) displays a remarkable degree of spontaneous anatomical and functional regeneration. To explore extrinsic mechanisms of neural repair, we carried out single-cell analysis of naïve mouse sciatic nerve, peripheral blood mononuclear cells, and crushed sciatic nerves at 1 day, 3 days, and 7 days following injury. During the first week, monocytes and macrophages (Mo/Mac) rapidly accumulate in the injured nerve and undergo extensive metabolic reprogramming. Proinflammatory Mo/Mac with a high glycolytic flux dominate the early injury response and rapidly give way to inflammation resolving Mac, programmed toward oxidative phosphorylation. Nerve crush injury causes partial leakiness of the blood-nerve barrier, proliferation of endoneurial and perineurial stromal cells, and entry of opsonizing serum proteins. Micro-dissection of the nerve injury site and distal nerve, followed by single-cell RNA-sequencing, identified distinct immune compartments, triggered by mechanical nerve wounding and Wallerian degeneration, respectively. This finding was independently confirmed with
Identifiants
pubmed: 36515985
doi: 10.7554/eLife.80881
pii: 80881
pmc: PMC9829412
doi:
pii:
Substances chimiques
SARM1 protein, mouse
0
Cytoskeletal Proteins
0
Armadillo Domain Proteins
0
Banques de données
GEO
['GSE198582']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : T32 GM113900
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA185000
Pays : United States
Informations de copyright
© 2022, Zhao, Huffman, Hafner et al.
Déclaration de conflit d'intérêts
XZ, LH, HH, MA, MF, AK, RK, CF, RP, CJ, DK, RK, LY, JT, DG, RG No competing interests declared, GC Except for Gabriel Corfas, the authors declare no competing financial or non-financial interests. Gabriel Corfas is a scientific founder of Decibel Therapeutics; he has an equity interest in and has received compensation for consulting. The company was not involved in this study
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