A Mycobacterium tuberculosis fingerprint in human breath allows tuberculosis detection.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
14 12 2022
14 12 2022
Historique:
received:
25
03
2022
accepted:
05
12
2022
entrez:
14
12
2022
pubmed:
15
12
2022
medline:
17
12
2022
Statut:
epublish
Résumé
An estimated one-third of tuberculosis (TB) cases go undiagnosed or unreported. Sputum samples, widely used for TB diagnosis, are inefficient at detecting infection in children and paucibacillary patients. Indeed, developing point-of-care biomarker-based diagnostics that are not sputum-based is a major priority for the WHO. Here, in a proof-of-concept study, we tested whether pulmonary TB can be detected by analyzing patient exhaled breath condensate (EBC) samples. We find that the presence of Mycobacterium tuberculosis (Mtb)-specific lipids, lipoarabinomannan lipoglycan, and proteins in EBCs can efficiently differentiate baseline TB patients from controls. We used EBCs to track the longitudinal effects of antibiotic treatment in pediatric TB patients. In addition, Mtb lipoarabinomannan and lipids were structurally distinct in EBCs compared to ex vivo cultured bacteria, revealing specific metabolic and biochemical states of Mtb in the human lung. This provides essential information for the rational development or improvement of diagnostic antibodies, vaccines and therapeutic drugs. Our data collectively indicate that EBC analysis can potentially facilitate clinical diagnosis of TB across patient populations and monitor treatment efficacy. This affordable, rapid and non-invasive approach seems superior to sputum assays and has the potential to be implemented at point-of-care.
Identifiants
pubmed: 36517492
doi: 10.1038/s41467-022-35453-5
pii: 10.1038/s41467-022-35453-5
pmc: PMC9751131
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7751Informations de copyright
© 2022. The Author(s).
Références
Microbiology (Reading). 2002 Oct;148(Pt 10):3049-3057
pubmed: 12368438
Nat Commun. 2021 Mar 11;12(1):1606
pubmed: 33707445
J Breath Res. 2017 Jan 09;11(1):016003
pubmed: 28068297
Chem Biol. 2006 Jan;13(1):39-47
pubmed: 16426970
J Biol Chem. 1997 Sep 12;272(37):23094-103
pubmed: 9287310
J Pharm Biomed Anal. 2015 Feb;105:134-149
pubmed: 25555262
Cell Host Microbe. 2010 Jul 22;8(1):68-76
pubmed: 20638643
Eur Respir J. 2014 Aug;44(2):537-40
pubmed: 24696110
Respirology. 2018 May;23(5):455-466
pubmed: 29457312
J Microbiol Methods. 2001 May;45(1):41-52
pubmed: 11295196
Sci Rep. 2020 May 6;10(1):7647
pubmed: 32376992
J Clin Med. 2019 Dec 31;9(1):
pubmed: 31906163
Lancet Infect Dis. 2020 May;20(5):607-617
pubmed: 32085847
J Lipid Res. 2011 Jun;52(6):1098-1110
pubmed: 21482713
Front Cell Infect Microbiol. 2015 Jan 12;4:187
pubmed: 25629008
Chem Biol. 2011 Dec 23;18(12):1537-49
pubmed: 22195556
J Clin Microbiol. 2013 Dec;51(12):4161-6
pubmed: 24108610
J Biol Chem. 2003 Aug 8;278(32):29880-9
pubmed: 12775723
Tuberculosis (Edinb). 2012 Jul;92(4):314-20
pubmed: 22647661
Chem Biol. 2014 Jan 16;21(1):67-85
pubmed: 24374164
ACS Infect Dis. 2020 Feb 14;6(2):291-301
pubmed: 31762254
Biochimie. 2003 Jan-Feb;85(1-2):153-66
pubmed: 12765785
Methods Mol Biol. 2015;1285:77-103
pubmed: 25779311
PLoS One. 2015 Dec 03;10(12):e0144088
pubmed: 26633829
Indian J Med Res. 2012 May;135(5):731-6
pubmed: 22771606
Respiration. 2007;74(3):329-33
pubmed: 17534131
Mol Microbiol. 2008 Jul;69(1):164-74
pubmed: 18466296
J Biol Chem. 2006 Jul 14;281(28):19512-26
pubmed: 16687411
Proteomics. 2015 Oct;15(19):3331-7
pubmed: 26201501
J Mol Biol. 2000 Jun 23;299(5):1353-62
pubmed: 10873458
Gates Open Res. 2017 Nov 7;1:11
pubmed: 29355225
Clin Infect Dis. 2017 Jan 15;64(2):111-115
pubmed: 28052967
Proc Natl Acad Sci U S A. 2014 Feb 25;111(8):2978-83
pubmed: 24516143
Mol Immunol. 2021 May;133:175-181
pubmed: 33743266
Chest. 2006 Nov;130(5):1541-6
pubmed: 17099035
ERJ Open Res. 2019 Feb 11;5(1):
pubmed: 30775376
Anal Chim Acta. 2013 Dec 17;805:1-18
pubmed: 24296139
J Immunol. 2018 May 1;200(9):3053-3066
pubmed: 29610143
Tuberculosis (Edinb). 2013 Mar;93(2):232-8
pubmed: 23127779
Proteomics. 2010 Sep;10(17):3190-202
pubmed: 20662102
Glycobiology. 2015 Nov;25(11):1163-71
pubmed: 26261090
J Biol Chem. 2000 Jan 7;275(1):677-84
pubmed: 10617666
PLoS One. 2016 Jan 25;11(1):e0146658
pubmed: 26807816
J Clin Microbiol. 2018 Nov 27;56(12):
pubmed: 30257899
Antioxidants (Basel). 2021 Oct 05;10(10):
pubmed: 34679707
J Biol Chem. 1993 Jun 15;268(17):12401-11
pubmed: 8509380
Chem Biol. 2012 Feb 24;19(2):218-27
pubmed: 22365605
Anal Chem. 2006 Dec 15;78(24):8543-8
pubmed: 17165851
Biochemistry. 2011 Oct 25;50(42):9135-47
pubmed: 21919534
Bioinformatics. 2020 May 1;36(10):3148-3155
pubmed: 32096818
JAMA Netw Open. 2019 Feb 1;2(2):e187617
pubmed: 30735231
Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5133-8
pubmed: 17360366
Tuberculosis (Edinb). 2021 Jan;126:102038
pubmed: 33316737
Trends Microbiol. 2022 Jul 30;:
pubmed: 35918247
J Clin Invest. 2011 Apr;121(4):1471-83
pubmed: 21364279
Lancet Respir Med. 2015 Mar;3(3):201-9
pubmed: 25726085
Immunol Allergy Clin North Am. 2018 Nov;38(4):667-678
pubmed: 30342587
Lancet Infect Dis. 2018 Jul;18(7):e199-e210
pubmed: 29580818