Substrate stiffness promotes dentinogenesis via LAMB1-FAK-MEK1/2 signaling axis.
cell-material interfaces
dentinogenesis
focal adhesions
mechanical cues
mechanotransduction
Journal
Oral diseases
ISSN: 1601-0825
Titre abrégé: Oral Dis
Pays: Denmark
ID NLM: 9508565
Informations de publication
Date de publication:
15 Dec 2022
15 Dec 2022
Historique:
revised:
09
12
2022
received:
08
10
2022
accepted:
12
12
2022
pubmed:
16
12
2022
medline:
16
12
2022
entrez:
15
12
2022
Statut:
aheadofprint
Résumé
In vivo, the principal function of mechanosensitive odontoblasts is to synthesize and secrete the matrix which then calcifies and forms reactive dentin after exposure to appropriate stimuli. This study aims to develop the influence of mechanical factors on dentinogenesis based on odontoblasts, which contribute to reparative dentin formation. We fabricated polydimethylsiloxane with different stiffnesses and seeded 17IIA11 odontoblast-like cells on the substrates in different stiffnesses. Cell morphology was detected by scanning electron microscope, and the mineralization phenotype was detected by alkaline phosphatase staining and alizarin red staining, while expression levels of dentinogenesis-related genes (including Runx2, Osx, and Alp) were assayed by qPCR. To explore mechanism, protein distribution and expression levels were detected by immunofluorescent staining, Western blotting, and immunoprecipitation. In our results, during dentinogenesis, 17IIA11 odontoblast-like cells appeared better extension on stiffer substrates. The binding between LAMB1 and FAK contributed to converting mechanical stimuli into biochemical signaling, thereby controlling mitogen-activated protein kinase kinase 1/2 activity in stiffness-driven dentinogenesis. The present study suggests odontoblast behaviors can be directly regulated by mechanical factors at cell-material interfaces, which offers fundamental mechanism in remodeling cell microenvironment, thereby contributing to physiological phenomena explanation and tissue engineering progress.
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : China Postdoctoral Science Foundation
ID : 2019M663525
Organisme : National Natural Science Foundation of China
ID : 82001020
Organisme : National Natural Science Foundation of China
ID : 81873708
Organisme : National Natural Science Foundation of China
ID : 82001062
Organisme : Science and Technology Project of Sichuan Province
ID : 2021YFG0238
Organisme : Science and Technology Project of Sichuan Province
ID : 2021YFH0185
Organisme : West China School/Hospital of Stomatology Sichuan University
ID : RCDWJS2021-13
Organisme : Sichuan University
Organisme : West China Hospital
Informations de copyright
© 2022 Wiley Periodicals LLC.
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