Transposon insertion sequencing analysis unveils novel genes involved in luxR expression and quorum sensing regulation in Vibrio alginolyticus.

Animals Vibrio alginolyticus / metabolism Quorum Sensing / genetics Gene Expression Regulation, Bacterial Vibrio Infections / veterinary Trans-Activators Bacterial Proteins / genetics
LuxR Temperature Transposon insertion sequencing (Tn-seq) Vibrio alginolyticus

Journal

Microbiological research
ISSN: 1618-0623
Titre abrégé: Microbiol Res
Pays: Germany
ID NLM: 9437794

Informations de publication

Date de publication:
Feb 2023
Historique:
received: 04 10 2022
revised: 20 10 2022
accepted: 24 10 2022
pubmed: 16 12 2022
medline: 21 12 2022
entrez: 15 12 2022
Statut: ppublish

Résumé

Vibrio alginolyticus is an important conditional pathogen of fish, shrimp, shellfish, and other marine aquaculture animals that causes huge economic losses to the marine aquaculture industries. Temperature has a significant influence on its quorum sensing (QS) system, which is essential for its various physiological functions. Using transposon insertion sequencing (Tn-seq) technology, we identified 218 putative regulatory factors of LuxR, the master regulator of QS in V. alginolyticus. In addition to established regulators, novel regulatory factors involved in LuxR expression are related to multiple processes. OmpH, 00189, TolC, VscY, and NirD are validated upstream regulatory factors of LuxR. Interestingly, OmpH and 00189 repress luxR expression at lower temperatures and activate its expression at higher temperatures. In contrast, TolC, VscY, and NirD enhance luxR expression at lower temperatures but suppress it at higher temperatures. Moreover, the abovementioned regulators are essential for QS-associated phenotypes, including Asp yields, motility, and biofilm formation, in temperature-dependent or temperature-independent manners. Thus, these novel regulators appear to relay various physiological signals in addition to temperature, effecting population phenotype modifications via QS regulation and warranting future investigation into the underlying mechanisms of opportunistic outbreaks of vibriosis.

Identifiants

DOI: 10.1016/j.micres.2022.127243 PMID: 36521340
pubmed: 36521340
pii: S0944-5013(22)00283-X
doi: 10.1016/j.micres.2022.127243
pii:
doi:

Substances chimiques

Trans-Activators 0
Bacterial Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

127243

Informations de copyright

Copyright © 2022 Elsevier GmbH. All rights reserved.

Déclaration de conflit d'intérêts

Conflict of interest disclosure The authors declare no conflict of interest.

Auteurs

Yibei Zhang (Y)

State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, Shanghai 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China.

Xiao Wu (X)

State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, Shanghai 200237, China.

Jingxiao Cai (J)

State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, Shanghai 200237, China.

Mo Chen (M)

State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, Shanghai 200237, China.

Jun Zhang (J)

State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, Shanghai 200237, China.

Shuai Shao (S)

State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, Shanghai 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China.

Yuanxing Zhang (Y)

Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519000, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China.

Yue Ma (Y)

State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, Shanghai 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China. Electronic address: mymarine@ecust.edu.cn.

Qiyao Wang (Q)

State Key Laboratory of Bioreactor Engineering, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, East China University of Science and Technology, Shanghai 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai 200237, China. Electronic address: oaiwqiyao@ecust.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Transposon insertion sequencing analysis...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Vibrionaceae › Vibrio › Vibrio alginolyticus › Transposon insertion sequencing analysis...
questionsmedicales.fr › Phénomènes microbiologiques › Interactions microbiennes › Détection du quorum › Transposon insertion sequencing analysis...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Transposon insertion sequencing analysis...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Vibrio › Transposon insertion sequencing analysis...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales régulatrices ou accessoires › Transactivateurs › Transposon insertion sequencing analysis...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Transposon insertion sequencing analysis...