Sequence-specific capture and concentration of viral RNA by type III CRISPR system enhances diagnostic.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
15 12 2022
15 12 2022
Historique:
received:
18
03
2022
accepted:
05
12
2022
entrez:
15
12
2022
pubmed:
16
12
2022
medline:
22
12
2022
Statut:
epublish
Résumé
Type-III CRISPR-Cas systems have recently been adopted for sequence-specific detection of SARS-CoV-2. Here, we repurpose the type III-A CRISPR complex from Thermus thermophilus (TtCsm) for programmable capture and concentration of specific RNAs from complex mixtures. The target bound TtCsm complex generates two cyclic oligoadenylates (i.e., cA
Identifiants
pubmed: 36522348
doi: 10.1038/s41467-022-35445-5
pii: 10.1038/s41467-022-35445-5
pmc: PMC9751510
doi:
Substances chimiques
DNA
9007-49-2
Endonucleases
EC 3.1.-
RNA, Viral
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
7762Subventions
Organisme : NIAID NIH HHS
ID : K99 AI171893
Pays : United States
Organisme : NIGMS NIH HHS
ID : K99 GM147842
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM128836
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM134867
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2022. The Author(s).
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