A Monoclonal Antibody Targeting C-Terminal Domain of Transmissible Gastroenteritis Virus Spike Protein.

Swine Animals Mice Transmissible gastroenteritis virus Antibodies, Monoclonal Spike Glycoprotein, Coronavirus Blotting, Western Gastroenteritis, Transmissible, of Swine
C-terminal domain TGEV monoclonal antibody spike

Journal

Monoclonal antibodies in immunodiagnosis and immunotherapy
ISSN: 2167-9436
Titre abrégé: Monoclon Antib Immunodiagn Immunother
Pays: United States
ID NLM: 101590955

Informations de publication

Date de publication:
Dec 2022
Historique:
pubmed: 17 12 2022
medline: 31 12 2022
entrez: 16 12 2022
Statut: ppublish

Résumé

The structure and function of the C-terminus domain (CTD) of porcine transmissible gastroenteritis virus (TGEV) spike protein remain largely unknown, thereby a specific monoclonal antibody (MAb) allows us to fully understand this domain. In this study, we developed a murine MAb against CTD of TGEV spike protein, as evidenced by the results of indirect fluorescent assay, Western blotting, and fluorescence-activated cell sorter. Further study showed that the MAb is able to exclusively recognize a 12-residue peptide (FKNVSDGVIYSV) derived from CTD of TGEV spike protein. This MAb can be used to elucidate the potential function of CTD of TGEV spike in virus attachment and entry, and warrants further intensive investigation.

Identifiants

DOI: 10.1089/mab.2022.0030 PMID: 36525526
pubmed: 36525526
doi: 10.1089/mab.2022.0030
doi:

Substances chimiques

Antibodies, Monoclonal 0
Spike Glycoprotein, Coronavirus 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

328-332

Auteurs

Na Liu (N)

College of Life Science and Technology, Wuhan University of Bioengineering, Wuhan, China.

Yaoming Li (Y)

College of Life Science and Technology, Wuhan University of Bioengineering, Wuhan, China.
Hubei Engineering Research Center of Viral Vector, Applied Biotechnology Research Center, Wuhan University of Bioengineering, Wuhan, China.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › A Monoclonal Antibody Targeting C-Terminal...
questionsmedicales.fr › Eucaryotes › Animaux › A Monoclonal Antibody Targeting C-Terminal...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › A Monoclonal Antibody Targeting C-Terminal...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Alphacoronavirus › Alphacoronavirus-1 › Virus de la gastroentérite transmissible › A Monoclonal Antibody Targeting C-Terminal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › A Monoclonal Antibody Targeting C-Terminal...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines de l'enveloppe virale › Protéines de fusion virale › Glycoprotéine de spicule des coronavirus › A Monoclonal Antibody Targeting C-Terminal...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Technique de Western › A Monoclonal Antibody Targeting C-Terminal...
questionsmedicales.fr › Maladies de l'animal › Maladies des porcs › Gastroentérite transmissible du porc › A Monoclonal Antibody Targeting C-Terminal...