The mechanism and significance of the conversion of xanthine dehydrogenase to xanthine oxidase in mammalian secretory gland cells.
Journal
Redox biology
ISSN: 2213-2317
Titre abrégé: Redox Biol
Pays: Netherlands
ID NLM: 101605639
Informations de publication
Date de publication:
02 2023
02 2023
Historique:
received:
04
11
2022
revised:
03
12
2022
accepted:
05
12
2022
pubmed:
17
12
2022
medline:
18
1
2023
entrez:
16
12
2022
Statut:
ppublish
Résumé
The conversion of xanthine dehydrogenase (XDH) to xanthine oxidase (XO) occurs only in mammalian species. In fresh bovine milk, the enzyme exists predominantly as the oxidase form, in contrast to various normal organs where it is found primarily as the dehydrogenase: the mechanism of conversion to the oxidase in milk remains obscure. A systematic search for the essential factors for conversion from XDH to XO has been performed within fresh bovine milk using the highly purified dehydrogenase form after removal endogenous oxidase form by fractionation analysis. We find that conversion to the oxidase form requires four components under air: lactoperoxidase (LPO), XDH, SCN
Identifiants
pubmed: 36525890
pii: S2213-2317(22)00345-7
doi: 10.1016/j.redox.2022.102573
pmc: PMC9760657
pii:
doi:
Substances chimiques
Xanthine Dehydrogenase
EC 1.17.1.4
Xanthine Oxidase
EC 1.17.3.2
Xanthines
0
Disulfides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
102573Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest There are no conflicts of interest to declare.